For Professionals in Research
The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry. It can be used with mouse and human cells during cell staining with cyanine-containing and cyanine-like tandem fluorochrome-conjugated antibodies.
Integrating the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer into standard flow cytometry staining protocols is simple – add the reagent to either the cell suspension or antibody cocktail. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is compatible with commonly used buffers such as the BD Horizon™ Brilliant Stain Buffer, BD Pharmingen™ Mouse Fc Block™ and BD Pharmingen™ Human BD Fc Block™
The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer should be used when staining cells with cyanine-containing and cyanine-like tandem fluorochromes such as PE-Cy5, PE-Cy7, APC-Cy7, APC-H7 and PE-CF594. These fluorochromes can contribute to varying levels of background staining on monocytes and lymphocytes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces non-specific interactions of the fluorochrome with leucocytes, revealing true expression of your target markers.
BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on human leukocytes, while maintaining antibody-specific staining
When staining lysed whole blood with cell surface markers (CD279, CD3, CD19) conjugated to PE-Cy7, there is background staining on monocytes. Addition of the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer to the staining protocol reduces the nonspecific background staining derived from PE-Cy7 on monocytes but does not affect antibody-specific staining of CD279 (PD-1), CD3 and CD19 staining on lymphocytes.
BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on mouse leukocytes
Background staining with cyanine-containing and cyanine-like tandem fluorochromes can also be observed in mouse leukocytes. In addition, some cell types express Fcγ receptors that can also contribute to background staining by binding non-specifically to detection antibodies. This is commonly addressed by using reagents that block Fc receptors such as BD Pharmingen™ Mouse Fc Block™. BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer can be used in combination with Fc blocking reagents. Together, they help resolve true positive populations from false negatives derived from dye and Fc receptor-mediated staining.
When staining mouse bone marrow cells with PE-Cy7 antibody conjugates without blocking reagents, both lymphoid and myeloid populations show background staining. Treatment with BD Pharmingen™ Mouse Fc Block™ reduces some of the background staining, but non-specific binding of the fluorochrome is still present. Staining cells in the presence of both BD Pharmingen™ Mouse Fc Block™ and the BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer shows further reduced background across three different antibody isotypes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on leukocytes, while preserving the staining intensity of antibody-specific binding such as CD11b.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described. Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
BD-104875
The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry.
