BD Horizon™ NEXT Global Educational Tour
As the need to resolve more complex biological data increases, it is critical for scientists to remain up to date on the latest technologies, methodologies and biological insights to help optimize their science. The BD Horizon™ NEXT Global Education Tour will empower scientists with the latest high-parameter spectral flow cytometry information and new-to-world real-time imaging cell sorting technology. This program will demonstrate the critical steps required to optimize a 38-color human NK and T cell panel prior to its use to assess the immune system diversity across healthy donors.
Attendees will also be introduced to the cutting-edge technologies at the core of the first real-time fluorescence imaging spectral flow cytometer and cell sorter. We will demonstrate the unprecedented capability of isolating six subsets of cells identified by the 38-color spectral flow cytometry panel.
Presentation modules:
Optimize: Learn best practices for the design and execution of high-quality high-parameter spectral flow cytometry panels
In this module, we will show you how to balance assay complexity and the maintenance of biological resolution by applying a deep human phenotype database and good practices in panel design to identify and select fluorochrome combinations. This approach was used to optimize a deep 38-color human NK and T cell panel that will be presented for the very first time.
Deep Science: Exploring the diversity of immune systems through deep spectral immunotyping and cell sorting.
In this module, we will use the optimized 38-color human NK and T cell panel to demonstrate how experimental conditions (storage, freezing and sampling time) can affect biological resolution. The panel will ultimately be utilized to reveal the uniqueness of phenotype expression among healthy donors by highlighting donor-to-donor variability at high depth and challenging the concept of "normality", prior to the unprecedented cell sorting of six subpopulations using the next-generation imaging spectral flow cytometer.
Imaging Spectral Sorting: Learn about the cutting-edge technologies featured in first ever real-time imaging, spectral flow cytometer.
In this module, we'll show how our next-generation instruments will include image-enabled sorting and spectral flow cytometry technologies. Learn more about new imaging parameters and how to take sorting to new levels, paving the way for researchers to address previously impossible-to-answer questions.
Limited availability. Talk to your BD representative today.
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