Cell Biology

Sample Preparation

Immunoprecipitation

Immunoprecipitation with Antibody: Agarose Conjugates

Find protocols for cell lysate preparation under denaturing and native conditions, and step-by-step instructions for immunoprecipitation of antibody:agarose conjugates.

Immunoprecipitation with anti-Phosphotyrosine: Biotin Conjugates

Find protocols for cell lysate preparation under denaturing and native conditions, and step-by-step instructions for immunoprecipitation of anti-PTyr:Biotin complexes.

Immunoprecipitation with Soluble Antibodies

Find protocols for tissue sectioning and immunohistochemical staining of frozen sections.

Western Blotting

Western Blotting with Rabbit Polyclonal Antibodies

Find complete protocol for western blotting with rabbit polyclonal antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.

Western Blotting with Monclonal Antibodies

Get complete protocol for western blotting with monoclonal antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.

Western Blotting with Biotinylated Antibodies

Find complete protocol for western blotting with biotinylated antibodies – from sample preparation to gel electrophoresis to transfer and antibody incubation – step-by step instructions are included, along with some troubleshooting tips.

Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates