Description
This kit contains all the reagents required to run the BD Rhapsody™ Single Cell Analysis System or BD Rhapsody™ Express Single-Cell Analysis System 4 times. The kit must be run with a BD pre-designed or custom panel. Kit reagents can be combined with Sample Tags (Catalog No. 633781) for multiplexing of samples on one BD Rhapsody run and/or used with BD AbSeq™ antibody-oligonucleotides.
Preparation And Storage
Store the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit and BD Rhapsody™ cDNA Kit at –25°C to –15°C. Store the BD Rhapsody™ Cartridge Reagent Kit 2°C to 8°C and the BD Rhapsody™ Cartridge Kit at room temperature (15°C to 25°C ).
| Description | Quantity/Size | Part Number | |
|---|---|---|---|
| Targeted mRNA and AbSeq Amp Kit | 1 Each (1 ea) | 633774 | N/A |
| cDNA Kit | 1 Each (1 ea) | 633773 | N/A |
| Cartridge Kit | 1 Each (1 ea) | 633733 | N/A |
| Cartridge Reagent Kit | 1 Each (1 ea) | 633731 | N/A |
Development References (18)
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Alivernini S, MacDonald L, Elmesmari A, et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020; 26(8):1295-1306. (Methodology). View Reference
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Arroyo Hornero R, Georgiadis C, Hua P, et al. CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Commun Biol. 2020; 3(1):375. (Methodology). View Reference
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BD Rhapsody&trade. Single-Cell Analysis System Instrument User Guide (Doc ID: 214062). Available: http://www.bd.com/documents/guides/user-guides/GMX_BD-Rhapsody-Single-Cell-Analysis-System-Instrument_UG_EN.pdf .
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Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level. STAR Protoc. 2020; 1(2):100092. (Methodology). View Reference
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Ferreira RC, Castro Dopico X, Oliveira JJ, et al. Chronic Immune Activation in Systemic Lupus Erythematosus and the Autoimmune PTPN22 Trp620 Risk Allele Drive the Expansion of FOXP3+ Regulatory T Cells and PD-1 Expression. Front Immunol. 2019; 10:2606. (Methodology). View Reference
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Gertz ML, Chin CR, Tomoiaga D, et al. Multi-omic, Single-Cell, and Biochemical Profiles of Astronauts Guide Pharmacological Strategies for Returning to Gravity. Cell Rep. 2020; 33(10):108429. (Methodology). View Reference
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Hasegawa T, Kikuta J, Sudo T, et al. Identification of a novel arthritis-associated osteoclast precursor macrophage regulated by FoxM1. Nat Immunol. 2019; 20(12):1631-1643. (Methodology). View Reference
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Jensen-Cody SO, Flippo KH, Claflin KE, et al. FGF21 Signals to Glutamatergic Neurons in the Ventromedial Hypothalamus to Suppress Carbohydrate Intake. Cell Metab. 2020; 32(2):273-286. (Methodology). View Reference
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Jessica Ann Timms, Susann Winter, Steven Hargreaves, Donal P. McLornan, Uwe Platzbecker, Shahram Kordasti. Novel Simultaneous Single Cell mRNA and Protein Expression Profiling Identifies Distinct Treg and T Effector Signatures in the Bone Marrow of MDS Patients. Blood. 2019; 134(1):2980. (Methodology).
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Mair F, Erickson JR, Voillet V, et al. A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell Rep. 2020; 31(1):107499. (Methodology). View Reference
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Malkani S, Chin CR, Cekanaviciute E, et al. Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development. Cell Rep. 2020; 33(10):108448. (Methodology). View Reference
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Mohebiany AN, Ramphal NS, Karram K, et al. Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology. Cell Rep. 2020; 30(5):1585-1597. (Methodology). View Reference
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Sainson RCA, Thotakura AK, Kosmac M, et al. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression. Cancer Immunol Res. 2020; 8(12):1568-1582. (Methodology). View Reference
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Shamin Li, Yannick Simoni, Etienne Becht, et al. Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer. Cell Reports Medicine. 2020; 1(3):100039. (Methodology). View Reference
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Tang X, Zhang S, Peng Q, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020; 6(8):eaaz0374. (Methodology). View Reference
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Trzupek D, Dunstan M, Cutler AJ, et al. Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis. Genome Med. 2020; 12(1):55. (Methodology). View Reference
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Wagar LE, Salahudeen A, Constantz CM, et al. Modeling human adaptive immune responses with tonsil organoids. Nat Med. 2021; 27(1):125-135. (Methodology). View Reference
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Yang Y, Liu F, Liu W, et al. Analysis of single-cell RNAseq identifies transitional states of T cells associated with hepatocellular carcinoma. Clin Transl Med. 2020; 10(3):e133. (Methodology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
