• Fluorochromes can be added by name, laser, range of excitation or range of emission. Select multiple fluorochromes from one set of search results or add additional fluorochromes by continuing to search and clicking Add.
• After a fluorochrome is selected, corresponding emission and excitation curves will be displayed. Deselect Ex or Em in the fluorochrome list to modify the view. Hover over an excitation or emission curve to highlight the individual spectrum. Alternatively, highlight one or more fluorochromes in the graph by selecting them from your list in the fluorochrome tab.
• Save fluorochrome combinations in one of two ways. If you are logged in to your BD Biosciences account, click the Save Configuration button and find the file later under My Saved Items in the hamburger menu. Without an account/signing in, use the Export and Import option from the hamburger menu; refer to the Save and share instructions below for more details.
• Check back often as new fluorochromes will be added periodically.

TIP: Click on the information icon in the fluorochrome list to view basic fluorochrome information and access our catalog.

• To zoom in to a particular area on the graph, use your cursor (+) to click and drag over the area of interest. Click Reset Zoom above the graph to return to the default view.

• Save and share spectral graph images, fluorochrome combinations and customized instrument configurations.
  • Without logging in, you can export and import fluorochrome lists and customized instrument configurations as .csv files. These can be saved or shared locally and imported later using the Import option. Spectral graph images can be downloaded as a variety of file types that can be saved locally.
    
  • If you are logged in to your BD Biosciences account, you can save fluorochrome lists and instrument configurations as user-specific files within your account. These can be loaded from the My Saved Items section of the hamburger menu.

• Fluorochromes can be added by name, laser, range of excitation or range of emission. Select multiple fluorochromes from one set of search results or add additional fluorochromes by continuing to search and clicking Add.
• After a fluorochrome is selected, corresponding emission and excitation curves will be displayed. Deselect Ex or Em in the fluorochrome list to modify the view. Hover over an excitation or emission curve to highlight the individual spectrum. Alternatively, highlight one or more fluorochromes in the graph by selecting them from your list in the fluorochrome tab.
• Save fluorochrome combinations in one of two ways. If you are logged in to your BD Biosciences account, click the Save Configuration button and find the file later under My Saved Items in the hamburger menu. Without an account/signing in, use the Export and Import option from the hamburger menu; refer to the Save and share instructions below for more details.
• Check back often as new fluorochromes will be added periodically. A red X beside a chosen fluorochrome denotes that spectral signature data is not yet available and it can only be displayed in the combined or stacked graph views. Likewise, similarity and complexity values will not be available.

Tip: Click on the information icon in the fluorochrome list to view basic fluorochrome information and access our catalog.

• When a spectral instrument configuration is selected, the Similarity & Complexity matrix option becomes available to the right of the drop-down menu located above the graph. Click to view the matrix in a pop-up window.

Tip: You can export the matrix for future reference in the available file formats by using the Export button at the bottom right of the pop-up window.

• Save and share spectral graph images and fluorochrome combinations.
  • Without logging in, you can export and import fluorochrome lists as .csv files. These can be saved or shared locally and imported later using the Import option. Spectral graph images can be downloaded as a variety of file types that can be saved locally.
  • If you are logged in to your BD Biosciences account, you can save fluorochrome lists as user-specific files within your account. These can be loaded from the My Saved Items section of the hamburger menu.
  • Additionally, you can export the Similarity or Complexity matrix for future reference in the available file formats by using the Export button at the bottom right of the pop-up window.