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BD Pharmingen™ Purified Mouse Anti-c-Jun (pS63)
Clone 2/c-Jun/(S63) (RUO)




Western blot analysis of c-Jun (pS63) in human vascular endothelium. Lysates from control (left panel) and calyculin A-treated (right panel) EA-hy 926 cells (Edgell, McDonald, Graham, 1983) were probed with purified Mouse Anti-c-Jun (pS63) monoclonal antibody at concentrations of 0.063, 0.032, and 0.016 m g/ml (lanes 1, 2, and 3, respectively). c-Jun (pS63) is identified as a band of 39 kDa in the treated cells.


BD Pharmingen™ Purified Mouse Anti-c-Jun (pS63)

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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The a ctivator p rotein transcription factor (AP-1) was identified as a protein that recognizes specific sequences in the cis -control regions of the SV40 virus and the human metallothionein IIA gene. AP-1 is composed of protein products of two different gene families: jun and fos . The AP-1 transcription factor is either a homodimer of Jun proteins or a heterodimer of Jun and Fos proteins. The transcriptional activity of Jun is enhanced by phosphorylation of serines 63 (S63) and 73 in its activation domain. Phosphorylation at both sites is necessary for stimulating the activating function of Jun. Jun is phosphorylated by JNK protein kinases that are activated by the same signals that potentiate Jun activity.
The 2/c-Jun/(S63) monoclonal antibody recognizes the phosphorylated S63 of c-Jun.
Development References (7)
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Behrens A, Sibilia M, Wagner EF. Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation. Nat Genet. 1999; 21:326-329. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)).
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Dunn C, Wiltshire C, MacLaren A, Gillespie DA. Molecular mechanism and biological functions of c-Jun N-terminal kinase signalling via the c-Jun transcription factor. Cell Signal. 2002; 14(7):585-593. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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Lamph WW, Wamsley P, Sassone-Corsi P, Verma IM. Induction of proto-oncogene JUN/AP-1 by serum and TPA. Nature. 1988; 334(6183):629-631. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001; 20(19):2390-2400. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)).
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Smeal T, Binetruy B, Mercola D, et al. Oncoprotein-mediated signalling cascade stimulates c-Jun activity by phosphorylation of serines 63 and 73. Mol Cell Biol. 1992; 12(8):3507-3513. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)).
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Weiss C, Bohmann D. Deregulated repression of c-Jun provides a potential link to its role in tumorigenesis. Cell Cycle. 2004; 3(2):111-113. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)).
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Wisdom R, Johnson RS, Moore C. c-Jun regulates cell cycle progression and apoptosis by distinct mechanisms. EMBO J. 1999; 18(1):188-197. (Biology: Western blot, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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