Description
Interleukin-1 β (IL-1 β ) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The mature form of human IL-1 β is a 17 kD protein containing 153 amino acid residues, representing amino acids A117 through S269 of the IL-1 β precursor protein. The BD OptEIA™ Human IL-1 β ELISA Kit is for the quantitative determination of Human IL-1 β in serum, plasma and cell culture supernatant.
Components Quantity Antibody Coated Wells 2 plates (12 strips x 8 wells per plate) Detection Antibody 30 mL Standards 4 vials, lyophilized Enzyme Concentrate (250x) 150 µ l Enzyme Diluent 30 mL ELISA Dilution reagent 30 mL Wash Concentrate (20x) 100 mL TMB One-Step Substrate Reagent 30 mL Stop Solution 13 mL Plate Sealers 4 sheets
Preparation And Storage
Store unopened reagents at 2-8°C. do not use reagents after expiration date, or if turbidity is evident. Before use, bring all reagents to room temperature (18-25°C). Immediately after use, return to proper storage conditions. Lyophilized standards are stable until expiration date. After reconstitution, immediately aliquot standard stock in polypropylene vials at 50 µl per vial anf freeze at -80°C for up to 6 months. If necessary, store at 2-8°C for up to 8 hours prior to aliquotting/freezing. Do not leave reconstituted standard at room temperature.
| Description | Quantity/Size | Part Number | |
|---|---|---|---|
| Human IL-1β Standard | N/A | 51-9004474 | N/A |
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-22940-00
