Explore our broad portfolio of high-quality dyes and conjugates that can help expand your experimental design options. To power our BD FACSymphony™ family of instruments to do high-parameter flow cytometry, we offer small-scale conjugations between virtually any dye and any clone in our catalog. We also offer access to prototype dyes that are exclusive to BD, and we can conjugate our dyes to customer-provided materials.
Custom Conjugates
The BD catalog contains over 18,000 unique antibody-dye combinations for flow cytometry, including the BD Horizon Brilliant™ Reagents. However, we know that your research sometimes requires a unique combination that is not readily available. The BD customs program can provide any conjugation you need within our portfolio of fluorescent dyes and antibodies, with all the same configurable specifications.
Are you developing your own antibodies in house or sourcing antibody content that BD does not have? Send us your antibodies! BD Biosciences will conjugate your antibodies to our wide array of fluorochromes, including our BD Horizon Brilliant™ Dyes.*
To inquire about bulk quantities of a catalog product or purified antibodies, email us at BDB_Custom_Orders@BD.com.
Once you have submitted a request, please allow up to two business days to receive a preliminary quotation. Production lead times and minimum order quantities are dependent on the reagent you choose.
Format: Dried, Lyophilized, Liquid
Packaging: Tubes, Vials
Minimum order size: 0.05 mg if BD materials, 2 mg if customer provided material
*Not all isotypes and antibodies will apply
Below is the list of fluorochromes currently available for custom conjugation. Our team of experienced chemists can address any additional configuration or special requirements to deliver the product you need.
| Excitation Laser Line | Fluorochrome | Ex-max (nm) | Em-max (nm) | Relative Brightness |
|---|---|---|---|---|
| UV 355 nm |
BD Horizon™ BUV395 | 348 | 395 | |
| BD Horizon™ BUV496 | 350 | 496 | ||
| BD Horizon™ BUV563 | 350 | 564 | ||
| BD Horizon™ BUV615 | 350 | 615 | ||
| BD Horizon™ BUV661 | 350 | 660 | ||
| BD Horizon™ BUV737 | 350 | 735 | ||
| BD Horizon™ BUV805 | 351 | 803 | ||
| Violet 405 nm |
BD Horizon™ BV421 | 407 | 423 | |
| BD Horizon™ V450 | 405 | 450 | ||
| Pacific Blue™ | 404 | 455 | ||
| BD Horizon™ BV480 | 440 | 479 | ||
| BD Horizon™ V500 | 415 | 499 | ||
| BD Horizon™ BV510 | 405 | 512 | ||
| BD Horizon™ BV570*** | 407 | 573 | ||
| BD Horizon™ BV605 | 407 | 605 | ||
| BD Horizon™ BV650 | 406 | 649 | ||
| BD Horizon™ BV711 | 407 | 713 | ||
| BD Horizon™ BV75O | 409 | 754 | ||
| BD Horizon™ BV786 | 407 | 786 | ||
| Blue 488 nm |
BD Horizon™ BB515 | 490 | 515 | |
| Alexa Fluor™ 488 | 494 | 517 | ||
| FITC | 494 | 518 | ||
| BD Horizon™ RB545 | 496 | 545 | ||
| BD Horizon™ BB630-P2*** | 476 | 614 | ||
| BD Horizon™ BB660-P2*** | 476 | 660 | ||
| PerCP** | 481 | 675 | ||
| PerCP-Cy5.5** | 482 | 676 | ||
| BD Horizon™ BB700 | 476 | 695 | ||
| BD Horizon™ BB755-P*** | 478 | 755 | ||
| BD Horizon™ RB780 | 498 | 781 | ||
| Yellow-Green 561 nm |
PE* | 566 | 576 | |
| BD Horizon™ RY586 | 564 | 586 | ||
| BD Horizon™ PE-CF594* | 566 | 615 | ||
| PE-Cy5* | 566 | 670 | ||
| PE-Cy7* | 566 | 781 | ||
| Red 640 nm |
APC | 651 | 660 | |
| Alexa Fluor™ 647 | 653 | 669 | ||
| BD Horizon™ APC-R700 | 651 | 706 | ||
| BD Horizon™ Red 718 | 695 | 718 | ||
| Alexa Fluor™ 700 | 697 | 719 | ||
| APC-Cy7 | 651 | 779 | ||
| BD® APC H7 | 659 | 782 |
BUV, BD Horizon Brilliant™ UV; BV, BD Horizon Brilliant™ Violet; V, BD Horizon™ Violet; BB, BD Horizon Brilliant™ Blue; BYG, BD Horizon Brilliant™ Yellow-Green
*Excited by 488-nm, 532-nm and 561-nm lasers.
**Excited by 488-nm and 561-nm lasers.
***Available as custom.
For additional fluorochrome selection support, launch the BD® Spectrum Viewer Tool.
Prototypes of BD Horizon Brilliant™ Dyes (-P)
BD Life Sciences is committed to continuing to develop new BD Horizon Brilliant™ Dyes across various laser lines to improve the spectral properties of dyes and minimize the need for compensation in higher-order panels.
These prototype (-P) dyes have reached initial quality specifications but may undergo additional development that could result in minor performance changes. Any significant changes to the structure of the dye to optimize performance will be appropriately communicated to customers.
Class 1 Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation.
CF is a trademark of Biotium, Inc.
Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
