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Overview

Next-generation laser-specific flow cytometry research reagents


The family of BD Horizon RealYellow and BD Horizon RealBlue Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

 

Driven by next-generation BD dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

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      RB545
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      BD Horizon RealBlue™ 545 (RB545) Reagents

      RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:

       

      • Minimal cross-laser excitation off the 561-nm yellow-green laser
      • Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

       

      Download the BD Horizon RealBlue™ 545 Brochure>

       
      RB545 vials
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      RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

      Performance

      BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

      RB545 Normalized Emission Filter Parameters
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      RB545 Spread Plots
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      Applications

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      BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

       

      BuffersResults
      BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
      CellBlox™ Blocking BufferCompatible
      BD Cytofix™ Fixation BufferStable at least 24 hours
      1% PFAStable at least 24 hours
      BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
      BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
      BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
      EDTA and HeparinCompatible
      BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
       
      RB613
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      RB705
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      RB744
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      RB780
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      RY586
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      RY610
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      RY775
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      “The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

       

      “The RB780 fluorochrome fits very well into the spectral gap of dyes primarily excited by the blue laser and can be separated beautifully from PE-Cy7 on instruments with blue and yellow-green lasers.”

       

      Dr. Florian Ebner
      Distinguished Lab Scientist
      Cancer Pharmacology and Disease Positioning
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      BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. CellBlox is a trademark of Thermo Fisher Scientific.