Cell Cycle Protocols
Sample Preparation
Human Cyclins
Staining Procedure for Flow Cytometric Detection of Human Cyclins
Get the standard protocol used for Quality Control testing of the anti-cyclin antibodies by flow cytometry. Procedure for fixation and staining are described. Some optimization may be necessary.
Ki-67 Detection
Flow Cytometry Staining Protocol for Detection of Ki-67
Get step-by-step staining protocol for flow cytometry detection of Ki-67, which can also be used for staining of a variety of other intracellular molecules, such as p53, PCNA, or p16[INK4].
BrdU Detection
Detection of BrdU Incorporation in DNA Synthesizing Cells
Find detailed protocol for detecting BrdU incorporation in newly synthesized DNA.
