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For Professionals in Research

Overview

The BD® OMICS-Guard Sample Preservation Buffer is developed and optimised to address the need for high-quality biological sample preservation over an extended period of time when samples cannot be processed at the same time or location.

 

  • Provides a stress-free, one-step preservation protocol with minimum hands-on time
  • Optimised to preserve cells for a variety of downstream transcriptomic, proteomic and multiomic applications, including RNA-seq, CITE-seq, flow cytometry and qPCR​
  • Protects cell viability and preserves different cell populations in your samples for up to 72 hours at 4 °C
  • Developed and tested across multiple sample types: PBMCs and tissue samples
  • Available in two, easy-to-use formats: 50 mL bottle or 12 x 1 mL vials

 

Download our datasheet to learn more about the BD® OMICS-Guard Sample Preservation Buffer.

performance1

One-Step Sample Preservation Workflow

 

 Single-Cell SuspensionTissue

Recommended Usage

1 to 10 million cells per 1 mL BD® OMICS-Guard Preservation Buffer

30 to 50 mg of tissue per 20 mL BD® OMICS-Guard Preservation Buffer

Storage Temperature

4 °C

Storage Duration

Up to 72 hours

 
  1. Collect single cells/dissociated single cells in suspension and spin at 400 x g for 5 minutes.

  2. Discard the supernatant and resuspend the cells in the BD® OMICS-Guard Preservation Buffer per the recommended usage listed above
    Note: Cells can be stored in Eppendorf tubes or equivalent.

  3. Place the cells at 4 °C for up to 72 hours.

  4. After storage, spin the cells at 800 x g for 5 minutes and discard the supernatant to remove the BD® OMICS-Guard Preservation Buffer. No further washing is required.

  5. Resuspend the cells in desired buffer for downstream applications.
  1. Section tissues into pieces and immediately put into the BD® OMICS-Guard Preservation Buffer.
    Note: aim for sections that are approximately 50 mg in weight.

  2. Place the tissue at 4 °C for up to 72 hours.

  3. After storage, dissociate the tissue by the desired method for single-cell application, spin the cells at 800 x g for 5 minutes, and discard the supernatant to remove the BD® OMICS-Guard Preservation Buffer. No further washing is required.

  4. Resuspend the cells in desired buffer for downstream applications.

Modifications to single-cell capture on the BD Rhapsody™ Single-Cell Analysis System

For cells/tissues preserved in BD® OMICS-Guard Preservation Buffer, change the lysis conditions in the single-cell capture workflow from 2 minutes to 5 minutes lysis time.

Improve Ab-Oligo Signal with the BD® AbSeq Enhancer Kit for BD® OMICS-Guard Buffer-Preserved Samples in CITE-seq

 

If staining with BD® AbSeq Antibody-Oligos after BD® OMICS-Guard Buffer preservation, use the BD® AbSeq Enhancer Kit to reduce nonspecific binding events and enhance the AbSeq signal.

 

Applications

  

   

For Research Use Only. Not for use in diagnostic or therapeutic procedures. Refer to Manufacturer's instructions for use and related user manuals and technical sheets before using this product as described. Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparison are not made against non-BD technologies, unless otherwise noted.

Alexa Fluor is a trademark of Life Technologies Corporation. CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.