For Professionals in Research
The BD® Flex Single-Cell Multiplexing Kits (BD® Flex SMK) utilise a two-step labelling approach to enable multiplexing for various types of single-cell samples. Every antibody-oligo in the BD® Flex SMK has a unique sample oligo barcode conjugated to an anti-R-phycoerythrin (PE), clone E31-1459, antibody. This approach allows you to select any PE-conjugated primary antibody with which to tag your samples of interest, regardless of species or cell type.
Features
Support higher sample throughput for larger cohort studies | Multiplex up to 24 samples per lane to minimise batch effect, while maintaining high sample tagging sensitivity and specificity |
Empower single-cell sample multiplexing for various species and cell types | BD® Flex SMK enables flexibility in choosing primary antibodies with which to tag your samples |
Reduce experimental cost with lower reagent cost per sample | Four separately sold kits each providing six samples tags, will take care of your small-scale study and help you save on the total BD Rhapsody™ System experiment when you multiplex more samples |
One format fits all BD Rhapsody™ Assays | BD® Flex SMK works seamlessly with BD Rhapsody™ Assays (transcriptome, cell surface protein, and immune repertoire profiling) on the BD Rhapsody™ Single-Cell Analysis System |
Increase cell number analysed with improved data quality | Load up to 40K pooled cells per lane and remove erroneous data from cell multiplets originating from multiple samples for cleaner single-cell multiomics data analysis |
Multiplexing 24 single-cell samples in one BD Rhapsody™ System run
t-SNE analysis of single-cell gene expression profile with 24 Sample Tags in the BD® Flex Single-Cell Multiplexing Kits and BD Rhapsody™ Human Immune Response Panel. Cells are clustered based on 399 genes in the BD Rhapsody™ Human Immune Response Panel. The three expected, phenotypically distinct population clusters corresponding to human B cell (Ramos, blue cells), T cell (Jurkat, yellow and green cells), and monocytes (THP1, red and brown cells) are obtained. High sample multiplexing sensitivity (>99%) and specificity (>95%) are achieved with 24 Sample Tags. Putative multiplet clusters are identified and removed by the BD® Flex SMK.
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Brochure
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Product Data Sheet
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Technical Note
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described. Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
