Fragment-Seq: A Spatial Transcriptomics Method to Study Tissue Microenvironments at Single-Cell Resolution
Spatial transcriptomics is a rapidly evolving field aiming to capture transcriptomic features and spatial relationships of cells within tissues. Current methods have limitations such as the need for prior knowledge for targeted panel assembly, lack of single-cell resolution, or low throughput.
Targeted multiomics: visualise transcript and protein data together
Discrepancies between low-abundance transcripts and proteins make data visualisation challenging.
NSCLC scRNA-seq Atlas shows variety and versatility of neutrophils in non-small cell lung cancer
Non-small cell lung cancer (NSCLC) contains a diversity of histological subtypes and specific mutational signatures.
Antigen-specific B cells and chronic infection: The OMIP panel
Originating in the bone marrow, antigen-specific B cells are critical in infection prevention and control through antibody production, professional antigen presentation and cytokine secretion.
The first OMIP panel to profile NK cells from tumour tissue
Discrepancies between low-abundance transcripts and proteins make data visualisation challenging.
Depolarised mitochondria in CD8+ TILs induce T-cell exhaustion
Mitochondria play a key role in sustaining the metabolic fitness of cells in response to metabolic perturbations by increased mitochondrial fusion and biogenesis.
New phenotyping of human dendritic cells and monocyte cells
Recent technological developments in single-cell analysis have significantly advanced our understanding of the subset heterogeneity of antigen-presenting cells.
Extricating Human Tumour Immune Alterations from Tissue Inflammation
Antigen-presenting cells (APSs) and T cells are present in many solid tumour types, where they are critical determinants of tumour development and disease outcome.
The Role of Immune Checkpoint Receptors in Cancer Immunity
The human immune system fights not only external pathogens but also clears internal abnormalities like dying and cancer cells.
High-Throughput and High-Dimensional Single-Cell Analysis of Antigen-Specific CD8+ T Cells
T cells play a vital role in immunology by fighting invading pathogens, destroying tumor cells and orchestrating vaccine response.
Computational Sorting With HyperFinder, FlowJo™ Software and BD FACSDiva™ Software
The use of machine learning tools like tSNE, UMAP, CITRUS and CellCNN for the analysis of flow cytometry data may result in the discovery of novel cell types with a distinct pattern of surface markers.
Intracellular Flow Cytometry Analysis Reveals the Involvement of Prostaglandin in the Expansion of Regulatory Hematopoietic Cells in Type 1 Diabetes
The transplant of Haematopoetic Stem and Progenitor cells (HSPC) has been employed in the treatment of haematological malignancies.
Analysing Pediatric Immune Response to SARS-CoV -2 Using Flow Cytometry
The COVID-19 pandemic has impacted every country in the world. In adults, 10-20% of the patients develop severe or life-threatening disease.
A New 16-Color Flow Cytometry Panel Protocol for the Functional Phenotyping of Circulating Human Cytotoxic
T Cells and NK Cells
Building a panel for the intracellular staining of immune cells could pose several challenges. A protocol published recently in Cell Press STAR Protocols takes these into account and provides a detailed workflow for efficiently phenotyping circulating human cytotoxic T cells and NK cells.
Using Single-Cell Multiomics to Identify a Novel Arthritis-Associated Osteoclast Precursor Macrophages
The authors of this article (Hasegawa, et.al, Nat.Immun 2019) have studied the formation of osteoclasts, which play a crucial role in arthritic bone erosion.
CLL Phenotyping with BD Rhapsody™ Targeted Panels and BD® AbSeq Assays
Chronic Lymphoid Leukemia (CLL), the most common form of leukemia in adults, is often characterised by progressive accumulation of incompetent lymphocytes in blood and bone marrow.
Computational Sorting With HyperFinder, FlowJo™ Software and BD FACSDiva™ Software
The use of machine learning tools like tSNE, UMAP, CITRUS and CellCNN for the analysis of flow cytometry data may result in the discovery of novel cell types with a distinct pattern of surface markers.
Intracellular Flow Cytometry Analysis Reveals the Involvement of Prostaglandin in the Expansion of Regulatory Hematopoietic Cells in Type 1 Diabetes
The transplant of Haematopoetic Stem and Progenitor cells (HSPC) has been employed in the treatment of haematological malignancies. However, studies have shown that HSPCs also possess immunoregulatory properties, making them potentially attractive alternatives to immunosuppressive drugs for the treatment of autoimmune diseases.
Analysing Pediatric Immune Response to SARS-CoV -2 Using Flow Cytometry
The COVID-19 pandemic has impacted every country in the world. In adults, 10-20% of the patients develop severe or life-threatening disease. However, in children, most patients display only minor symptoms and so their contributions to spreading the SARS-CoV-2 virus is unclear.
BD Joins Consortium to Accelerate Technology Development and Lower the Costs in Cell and Gene Therapy Manufacturing
Wouldn’t it be wonderful if we could reach a point in medical history where disease can be cured without drugs?
AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
The BD™ AbSeq oligonucleotide-conjugated antibodies, when incorporated into a single-cell mRNA-sequencing experiment, can yield combined transcript and protein expression data.
Bifidobacteria-Mediated Immune System Imprinting Early In Life
There is mounting evidence to suggest that during the first 3 months of life, the infant gut microbiome is crucial for immune development. Specialised microbes in the infant gut that rely on breast milk for nutrients play a key role in immune-microbe interactions.
Single-Cell Genomic Approaches for Developing the Next Generation of Immunotherapies
In recent times, developments in genomics, proteomics and single-cell technologies, coupled with advances in allied areas like computing and antibody engineering have enabled significant progress in drug development.
High-Dimensional Flow Cytometry in COVID-19
The rapid spread of COVID-19 infections all over the world and the need to develop a vaccine has necessitated the understanding of the adaptive immune response to the SARS-CoV-2 virus.
High-Parameter Flow Cytometry for Deep Immune Profiling of COVID-19 Patients
The SARS-CoV-2 virus infection presents a range of clinical manifestations, ranging from mild or no symptoms to moderate to severe respiratory illnesses, such as ARDS (acute respiratory-distress syndrome) and multiorgan failure, to death. The innate immune defense mounted by the body involves pattern recognition receptors (PAR), which activate an array of transcription factors through a signal transduction pathway, ultimately resulting in the secretion of different interferons and chemokines.
Immunotyping of Natural Killer (NK) Cells in COVID-19 Patients Using High-Parameter Flow Cytometry
SARS-CoV-2 infection elicits a spectrum of immune responses, activating both the innate and adaptive immune systems. Whether these immune responses are targeted and controlled or misdirected and uncontrolled could determine the outcome of the disease. Recent studies using deep immunoprofiling have indicated that the nature of immune responses can also determine the severity of the disease.
Using single-cell multiomics to study stem cell differentiation
For the central nervous system, organoids demonstrate high-cell diversity, while reproducing more cell-cell interactions and developing to later stages than 2D cultures. However, one of the challenges of using brain organoids has been the lack of reproducability of differentiation.
