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Uncover up to 30 immune markers in a single experiment

The BD® AbSeq Immune Discovery Panel (IDP) is developed using our antibody-oligo based technology and consists of 30 different specificities against most major human immune markers lyophilized in a single tube. The BD® AbSeq IDP is tested to detect human immune markers and is designed to work on the BD Rhapsody™ Single-Cell Analysis System with BD Rhapsody™ Single-Cell RNA Assays and BD® Multiplexing Kits.
 

Find more information from the BD® AbSeq Immune Discovery Panel brochure.

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List of BD® AbSeq IDP Specificities

30 pre-titrated antibodies

 
SpecificityCloneOligo ID
CD3UCHT1
AHS0231
CD4SK3AHS0032
CD8SK1AHS0228
CD11c
B-Ly6
AHS0056
CD14
MPHIP9
AHS0037
CD16
3G8AHS0053
CD19
SJ25C1
AHS0030
CD25
2A3
AHS0026
CD27M-T271AHS0025
CD28L293AHS0138
 
SpecificityCloneOligo ID
CD45RA
HI100
AHS0009
CD56
NCAM16
AHS0019
CD62L
DREG-56
AHS0049
CD127
HIL-7R-M21
AHS0028
CD134
ACT35
AHS0013
CD137
4B4-1
AHS0003
CD161
HP-3G10
AHS0205
CD183 (CXCR3)
1C6/CXCR3
AHS0031
CD185 (CXCR5)RF8B2AHS0039
CD186 (CXCR6)13B 1E5AHS0148
 
SpecificityCloneOligo ID
CD196 (CCR6)11A9AHS0034
CD197 (CCR7)2-L1-AAHS0273
CD272J168-540
AHS0052
CD278DX29
AHS0012
CD279EH12.1AHS0014
CD357 (GITR)V27-580
AHS0104
CD366 (TIM-3)7D3
AHS0016
HLA-DRG46-6
AHS0035
IgDIA6-2AHS0058
IdMG20-127AHS0198

BD® AbSeq IDP Protocol

Follow these simple steps to use the BD® AbSeq IDP:
 

Steps for reconstituting the lyophilized IDP and staining cells
 

  1. Remove the IDP tube from the foil bag and bring up to room temperature for 5 minutes
  2. Make sure the pellet is located at the bottom of the tube
  3. Add 35 µL of nuclease-free water to the bottom of the tube and allow antibodies to reconstitute for 5 minutes at room temperature
  4. Store the reconstituted antibodies on ice until the cells are ready for staining
    Note: Reconstituted antibodies should be used within 24 hours.
  5. To make 2X AbSeq labeling master mix, add 65 µL of BD Pharmingen™ Stain Buffer to the solution to bring it to a total of 100 µL
  6. Mix with 100 µL cell suspension prepared following Single Cell Labeling with BD® AbSeq Ab-Oligos (Doc ID 214394 Rev. 1.0) and stain the cells on ice for 30 minutes
  7. Add 3 mL BD Pharmingen™ Stain Buffer (FBS) to labeled cells and pipette-mix
  8. Centrifuge each tube at 400 x g for 5 minutes
  9. Uncap each tube and invert to decant supernatant into biohazardous waste. Keep the tube inverted and gently blot on a lint-free wipe to remove residual supernatant from tube rim
  10. Repeat steps 7–9 twice more for a total of three washes
  11. Resuspend pellet in 620 µL cold BD Pharmingen™ Stain Buffer (FBS) from the BD Rhapsody™ Cartridge Reagent Kit and proceed to single-cell capture
     

See the Single Cell Analysis Workflow with BD Rhapsody™ Systems.

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To request a quote or place an order, email bdb_custom_orders@bd.com or contact your local BD sales representative.

 

For Research Use Only. Not for use in diagnostic or therapeutic procedures.