Product Details
Description
The BD Pharmingen™ Anti-Ig HRP Detection Kits are designed for immuno-histochemical staining of acetone-fixed frozen sections, formalin- or zinc-fixed paraffin sections, and cytospins/smears. The detection kit can be used with any purified primary antibody. Each kit includes a biotinylated anti-Ig antibody that is species-specific (anti-mouse, anti-rat or anti-hamster), antibody diluent, pre-diluted Streptavidin-horseradish peroxidase (SAv-HRP), and DAB substrate. The affinity-purified, polyclonal, biotinylated secondary antibody is adsorbed against multiple sera of related species, reducing non-specific staining, while providing maximum reactivity. Antibody diluent is a buffered solution containing protein stabilizers to enhance antibody reactivity, detergent for permeabilization, and blocking agents to reduce non-specific staining. The ultra-sensitive SAv-HRP is pre-diluted for convenience. DAB substrate is provided for signal development. Consistent results are assured with all these matched reagents. A comprehensive series of protocols, for immunostaining in different types of tissues, is provided with the kit.
Content Quantity Goat anti-hamster IgG Cocktail, biotinylated 0.5 mL Antibody Diluent Buffer 50 mL Streptavidin-HRP 20 mL DAB Buffer 20 mL DAB Chromogen 1.0 mL
Preparation And Storage
All the components of the Anti-Ig HRP Detection Kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
