Overview
BD immunoassay ELISA reagents contain antibody pairs that have each been developed to work together to quantitate soluble analytes in serum, plasma and tissue culture supernatants using sandwich ELISA.
The reagents:
- Come in three convenient sandwich ELISA formats and provide flexibility and cost efficiency for the analysis of tissue culture supernatants
- BD OptEIA™ Kits contain all necessary reagents and precoated 96-well plates for analysis
- BD OptEIA™ Sets include sufficient capture/detection reagents and standards for five or twenty 96-well plates
- Are composed of unlabeled capture antibodies, biotinylated detection antibodies and protein standards
- Offer a wide range of specificities and assay design flexibility with individual reagents as well as convenient set and kit formats
- Provide a cost-effective approach with single-vial reagents offering a wide range of specificities along with assay design flexibility to develop your own immunoassays
Features
RESOURCES AND TOOLS
Expand All
- Frequently Asked Questions
- Product Information Sheets
- Protocols
- Tools
- Blocking the Plate [ QuickTime ] [ Windows Media ]
- Capture Antibody Preparation and Coating [ QuickTime ] [ Windows Media ]
- Getting Started [ QuickTime ] [ Windows Media ]
- Standard Preparation and Handling [ QuickTime ] [ Windows Media ]
- Standard and Sample Addition [ QuickTime ] [ Windows Media ]
- Stopping the Reaction and Reading the Plate [ QuickTime ] [ Windows Media ]
- TMB Substrate Preparation and Addition [ QuickTime ] [ Windows Media ]
- Troubleshooting: High Background [ QuickTime ] [ Windows Media ]
- Troubleshooting: Low/No Signal [ QuickTime ] [ Windows Media ]
- Troubleshooting: Poor Standard Curve [ QuickTime ] [ Windows Media ]
- Troubleshooting: Well-to-Well Precision [ QuickTime ]
- Washing the Plates [ QuickTime ] [ Windows Media ]
- Working Detector Preparation [ QuickTime ] [ Windows Media ]
- Product List
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
QuickTime is a trademark of Apple Inc. Windows Media is a trademark of Microsoft Corporation.
23-23169-00