The panel design process has been broken up into six steps. At each step, you will be prompted to input information that is pertinent to your panel. The information you will be prompted to enter is information that you likely use when designing panels currently. The tool has information about fluorochromes brightness, instrument configurations and resolution impact so you do not have to enter that information. The tool takes all of this information into account to provide alerts for reagent selections that may negatively affect population resolution.
The more accurate the inputs are, the more likely the tool will be able to provide relevant solutions. It is also important to note that the tool will not be able to detect every possible reagent selection that may negatively affect resolution, so we cannot guarantee that the panel designed will perform optimally. Additionally, even good panels can have alert messages. This is especially true with higher-order panels for which it may be difficult to avoid spectral overlap between reagents. In this case, the tool will allow you to see which populations are most likely to be negatively affected by the reagent selections and tradeoffs can be assessed.
If you would like to get expert advice prior to running your panel or if you have questions about the data obtained from your panel, our Technical Support team is readily available; just email ResearchApplications@bd.com.