Description
The BD™ CBA Human Th1/Th2 Cytokine Kit (Catalog No. 550749) can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples. Vial label Reagent Quantity A1 Human IL-2 Capture Beads 1 vial, 0.8 mL A2 Human IL-4 Capture Beads 1 vial, 0.8 mL A3 Human IL-5 Capture Beads 1 vial, 0.8 mL A4 Human IL-10 Capture Beads 1 vial, 0.8 mL A5 Human TNF Capture Beads 1 vial, 0.8 mL A6 Human IFN-γ Capture Beads 1 vial, 0.8 mL B Human Th1/Th2 PE Detection Reagent 1 vial, 4 mL C Human Th1/Th2 Cytokine Standards 2 vials, 0.2 mL lyophilized D Cytometer Setup Beads 1 vial, 1.5 mL E1 PE Positive Control Detector 1 vial, 0.5 mL E2 FITC Positive Control Detector 1 vial, 0.5 mL F Wash Buffer 1 bottle, 130 mL G Assay Diluent 1 bottle, 30 mL H Serum Enhancement Buffer 1 bottle, 10 mL
Preparation And Storage
| Description | Quantity/Size | Part Number | |
|---|---|---|---|
| C) Human Th1/Th2 Cytokine Standards | N/A | 51-2428KC | N/A |
| E1) PE Positive Control Detector | N/A | 51-2429KC | N/A |
| B Human Th1/Th2 PE Detection Reagent | N/A | 51-2427KC | N/A |
| Human IFN-γ Capture Beads | 1 Each (1 ea) | 51-9005727 | N/A |
| Human TNF Capture Beads | 1 Each (1 ea) | 51-9005724 | N/A |
| Human IL-10 Capture Beads | 80 Tests (1 ea) | 51-9005740 | N/A |
| Human IL-5 Capture Beads | 1 Each (1 ea) | 51-9005718 | N/A |
| Human IL-4 Capture Beads | 1 Each (1 ea) | 51-9005715 | N/A |
| Human IL-2 Capture Beads | 80 Tests (1 ea) | 51-9005736 | N/A |
| D Cytometer Setup Beads | 1 Each (1 ea) | 51-9005731 | N/A |
| BD(tm) CBA Serum Enhancement Buffer (H) | N/A | 51-9002150 | N/A |
| G Assay Diluent | 1 Each (1 ea) | 51-2432KC | N/A |
| F Wash Buffer | 1 Each (1 ea) | 51-2431KC | N/A |
| E2 FITC Positive Control Detector | 1 Each (1 ea) | 51-9005734 | N/A |
| E1 PE Positive Control Detector | 1 Each (1 ea) | 51-9005732 | N/A |
Development References (13)
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Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
