Multicolor Flow Cytometry Panel Design

Multicolor flow cytometry enables the simultaneous analysis of multiple markers at the single-cell level. By using fluorochrome conjugated antibodies that target specific cellular markers, researchers can analyze various cell characteristics like surface antigens and intracellular proteins. The design of a flow cytometry panel can be challenging and requires an understanding of several factors that can influence panel performance such as the fluorochromes and the instrument being used.

Overview

Panel Design Video Guide

Resources

Tools

Interactive Human Cell Map Clone Comparison Tool BD^®Spectrum Viewer BD FACSelect™ Buffer Compatibility Tool BD^® Research Cloud FlowJo™ v10 Software

Webinars

Achieve: Putting It All Together: Panel Design Principles for Surface and Intracelluar Staining Sentinel Marker Approach to Multicolor Flow Cytometry: An Experimental Design that Uses Key Markers to Define Cell Subpopulations to Extend Instrument Capability Resolve: Managing Instrument Setup to Address Fluorescence Spillover and to Maximize Resolution Elevate: The Importance of Surface Antigen Density and Intracellular Epitope Availability in Choosing the Appropriate Fluorochrome/Antibody Combination Reveal: Implications of Relative Fluorochrome Brightness in Resolving the Population of Interest Ask the Experts—A Solid Foundation for Simpler and Smarter Panel Design Providing a Solid Foundation for Simpler Smarter Flow Cytometry Panel Design: Harmony Seminar Series A Solid Foundation for Simpler and Smarter Panel Design BD Panel Design Matters: Practical Aspects of Designing Flow Cytometry Panels Making Panel Design Easier in the Era of High-Dimensional Flow Cytometry Compensation Matters: Considerations When Designing Your Flow Cytometry Panels

Blog Articles

BD Harmony Webinars by Bob Balderas, BD Biosciences How Dyes Evolved: The History of the Modern Flow Cytometry Dye Panel Discussion on Validating Assays in Flow Cytometry High-Dimensional Flow Cytometry for Improved Data Analysis FlowJo University – The One Stop for All Your FlowJo™ and SeqGeq™ Software Training Needs

Ebooks

33C SE Panel Sheet | Immunophenotyping and cell isolation with high-parameter spectral flow cytometry BD Leukocyte Panel Blocks

E-learning Courses

Flow Cytometry Basics

Publications

Mahnke YD, Roederer M. Optimizing a multicolor immunophenotyping assay. Clin Lab Med. 2007;27(3): 469–485, v. doi: 10.1016/j.cll.2007.05.002
Mair F, Tyznik A. High-dimensional immunophenotyping with fluorescence-based cytometry: a practical guidebook. Methods Mol Biol. 2019;2032:1-29. doi: 10.1007/978-1-4939-9650-6_1
Nguyen R, Perfetto S, Mahnke YD, Chattopadhyay P, Roederer M. Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A. 2013;83(3):306-315. doi: 10.1002/cyto.a.22251
Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry. 2001;45(3):194-205. doi: 10.1002/1097-0320(20011101)45:3<194::aid-cyto1163>3.0.co;2-c

Terms and Conditions

Panel iterations must be verified by a BD Applications Specialist. Valid only on panels designed by BD Biosciences in the last 2 months. Swaps that constitute more than a 20% change of the total number of reagents must be approved by BD.

Recommendations given by representatives of BD in the context of the creation and setup of customer-specific applications and assays and the composition of antibody panels for use in flow cytometry assays are provided in a diligent manner. However, the Customer acknowledges that (i) such recommendations should not be treated as a substitute for the Customer’s own examination, (ii) BD does not make any promises nor guarantees that recommendations are accurate and complete and will meet the Customer’s present or future needs or will produce positive or specific results and (iii) Customer remains solely responsible for validating and deciding on any such recommendations.

BD, therefore, accepts no liability whatsoever in connection with any such recommendation and/or any results generated on the basis of such recommendations and, to the fullest extent permitted by law, expressly disclaims any and all warranties and any liability in this respect. In particular, the Customer assumes sole responsibility for the validation, use, selection, and suitability of the recommendations for its needs and objectives.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Flow Cytometers are Class 1 Laser Products