BD Horizon™ Human T Cell Backbone Panel

Expand your T cell research with confidence

The BD Horizon™ Human T Cell Backbone Panel consists of an optimized configuration of core markers specifically designed to expand your research with recommended fluorochrome drop-ins and enable flexibility in panel design without compromising resolution.

Fluorochromes were strategically selected so that up to five recommended drop-ins can be added with minimal impact to the backbone resolution and minimal panel design effort, allowing you to focus on marker selection.

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The BD Horizon™ Human T Cell Backbone Panel contains a set of markers that can be combined with up to five additional fluorochromes without impacting resolution

Marker	Clone	Fluorochrome
CD3	UCHT1	BV510
CD4	SK3	BV786
CD8	RPA-T8	R718
CD45RA	HI100	PE-Cy7
CD197 (CCR7)	2-L1-A	BV711

BD Horizon™ Brilliant Stain Buffer is included to provide optimal reagent performance.

Examples of recommended drop-in fluorochromes

Laser	Drop-in
Violet 405 nm	BV421/V450/Pacific Blue™
Blue 488 nm	FITC/BB515/Alexa Fluor™ 488/PE
Yellow-Green 561 nm	PE/RY586
Red 640 nm	APC/Alexa Fluor™ 647
UV 355 nm	BUV395

Drop-in reagents not included in backbone kit.

AF: Alexa Fluor™ Reagent

BB: BD Horizon Brilliant™ Blue Reagent, BUV: BD Horizon Brilliant™ Ultraviolet Reagent

BV: BD Horizon Brilliant™ Violet Reagent, R: BD Horizon™ Red Reagent

RY: BD Horizon RealYellow™ Reagent

The BD Horizon RealYellow™ (RY) 586 Fluorochrome can be used as an alternative to PE when excited off the yellow-green laser. Simultaneous use of PE off the blue laser and RY586 off the yellow-green laser is not recommended.

The BD Horizon™ Human T Cell Backbone Panel offers increased flexibility and fewer design challenges

Resolution

Clear resolution of five core markers defining major naive and memory subsets of CD4+ and CD8+ T cells

Flexible

Verified flexibility enabling deeper investigation of different facets of T cell biology (activation, differentiation, polarization, Treg immunophenotype)

Compatible

Compatible with buffers and protocols used for intracellular detection of cytokines, transcription factors or phosphorylated proteins

High Performance

Strategically designed to be complemented with up to five drop-in markers with no resolution impact to the backbone or the selected drop-ins
Verified protocols to ensure optimal performance
Consistent performance across instruments with different optical configurations

Performance

Performance of BD Horizon^TM Human T Cell Backbone Panel including drop-in configurations

Applications

Identification of FoxP3+ Treg subsets

A) The backbone panel was complemented with surface and intracellular markers conventionally used to identify human Treg subsets.
B) Clear resolution of all the subsets of interest was observed on fixed and permeabilized PMBCs from N = 3 healthy donors. Samples were acquired on a 4-laser BD FACSymphony™ A1 Cell Analyzer and analyzed with FlowJo™ Software v10.8.1.