BD FACSMelody™ Cell Sorter

Discover an approachable, affordable and automated 4-way cell sorting solution

Overview

The simple solution for consistent, quality results

The BD FACSMelody^™ Cell Sorter with newly added 4-way cell sorting:

Makes the complex world of flow cytometry and sorting accessible to more researchers
Offers advanced automation technology with BD FACSChorus^™ Software and guides you through the entire cell sorting process with its intuitive interface, on-screen instructions and tips and simple-to-read reports
Combines the proven and exclusive BD technology of high-end sorters with automation and simplified software
Helps in obtaining deep scientific insights, lab efficiency and cost savings

Learn more from the BD FACSMelody^™System brochure.

Features

The FACSMelody™ Cell Sorter is easy to learn and use with minimal training required for effective operation

Eliminate manual steps and simplify your workflow with smart automation

The system is typically ready in less than 17 minutes, maximizing uptime
Fluidic startup automatically de-bubbles the stream
Instrument settings, gates and sort settings are available and retrievable from saved experiments
Compensation is conveniently computed automatically from the results of compensation controls

Learn more about the BD FACSMelody™ Cell Sorter from the system brochure

The BD FACSMelody™ Cell Sorter saves time with simplified operation and maintenance and improves lab efficiency

Experience fast results to improve lab throughput, minimal downtime and cost savings

A streamlined workflow eliminates manual setup of the stream and monitoring of the sort, minimizing hands-on time and providing walk-away sorting capability.
Fixed alignment removes the lengthy procedure with alignment beads required at startup.
Instrument QC continuously monitors the system to track performance and predict problems such as laser failures before they occur. QC is not required each time the sorting nozzle is installed.
The sorter design facilitates fast troubleshooting, such as easy and quick replacement of the sorting nozzle, without a need to re-align the laser and stream or run QC beads.
The fixed flow cell and reusable nozzle eliminate the need to purchase daily consumables. This affordable solution allows in-house cell sorting, which can save money compared to outsourcing.

Learn more about the BD FACSMelody™ Cell Sorter from the system brochure.

The BD FACSMelody™ System maximizes signal generation, collection and detection and enables sorting and detection of rare and dim cells

Reliable performance designed to empower scientific discovery

The patented gel-coupled cuvette with fixed alignment ensures that lasers are precisely focused on the sample stream to generate optimal signal for clear resolution of dim cells and adjacent populations
Spatially separated laser beams ensure minimal optical background noise and improved sensitivity from each laser to resolve dim populations

Optimized system of reagents, software and hardware

Instrument configurations are optimized for bright BD Horizon Brilliant™ and BD OptiBuild™ Reagents.
Sweet Spot technology maintains sort stream stability so yield and purity are maintained.

Learn more about the BD FACSMelody™ Cell Sorter from the system brochure.

The BD FACSMelody™ Cell Sorter provides a comprehensive biosafety solution

We have partnered with The Baker Company to design a custom biosafety and sorting solution. Our solution is comprehensive and keeps your cell sorting activities running smoothly and safely, so you can focus on your research.

View the Microbiological Test Report for the BD FACSMelody™ Cell Sorter.

Here are the most recent biosafety and sorting guidelines, policies and publications for your reference.

Baker biosafety solution	Sorter + BSC measurements	Verified to meet biosafety standards for a Class II Type A2 biosafety cabinet	Verified for personnel and product protection	Microbiological test report	Meets ISAC guidelines for aerosol management
SterilGARD™ e3 Optimax for the BD FACSMelody™ Cell Sorter	53.8 x 34.5 x 91.9 to 98.4 in. (136.5 x 87.6 x 233.4 to 249.9 cm)	√	√	√	√

Applications

The BD FACSMelody™ Cell Sorter has demonstrated capabilities to sort various types of cells and cell subsets.

One workflow: Cell enrichment to single-cell transcriptome and proteome analysis

Watch the video describing how to gain insights into mRNA and protein expression by enriching cell populations using the BD FACSMelody^™ Cell Sorter.

Streamlining cell sorting and single-cell multiomics analysis

Discover the combined potential of cell sorting and single-cell analysis for deeper insights. Watch how the BD FACSMelody™ Cell Sorter and the BD Rhapsody™ Single-Cell Analysis System can streamline your research.

Will you need to preserve your samples before running your experiment? BD^® OMICS-Guard Sample Preservation Buffer can preserve the cell viability of your samples for up to 72 hours. Click here to get more information.

Multiomic Analysis of Treg heterogeneity for optimization of Treg-based cell therapies

BD Biosciences has launched this webinar, presenting BD Rhapsody^™ single cell isolation and preparation technology for proteomic and transcriptomic study of immune cells.

CD4+ regulatory T cells expressing FOXP3 (Tregs) play an essential role in maintaining self-tolerance. Treg cells play a key role in preventing autoimmune diseases and stimulating tolerance to allogeneic transplants. The use of Treg cells represents a promising innovative autoimmune disease and transplantation therapeutic strategy.

Treg cells are functionally and phenotypically heterogeneous when isolated from peripheral blood or after expansion for therapeutic purposes. The use of single-cell RNA sequencing allows us to analyze their transcriptome in detail. This allows us to identify different subgroups of Treg cells in their natural state and after expansion.

Prof. Makoto Miyara, MD, PhD is a Full Professor in Immunology. He is the coordinator of the reference laboratory for LED and antiphospholipid antibody syndrome at the APHP, Sorbonne-Université, Pitié-Salpêtrière. His specialty as a professional is to develop innovative diagnostic techniques, including AI use for pattern recognition and gene editing to detect autoantibodies. He completed his clinical training at Paris' Pitié-Salpêtrière hospital as an Internist. He leads a research group that focuses on manipulating tolerance mechanisms, primarily regulatory T cells, to offer innovative therapeutic strategies to promote allograft tolerance in transplants, restore immune homeostasis in autoimmune diseases, promote tissue regeneration or enhance antitumor immune responses. His main articles have been quoted more than 1500 times. He is the Lead Investigator of three ongoing Phase I clinical trials evaluating Treg cell therapy in liver, kidney and lung transplants.

Testimonial

“BD FACSMelody is very easy to use.”

Dr. Andrea L. Holme,

Senior Lecturer for Cytometry,

The University of Western Australia