Minimize spectral overlap/spread and clearly distinguish cells populations with low antigen density. We offer a wide variety of fluorochromes across multiple lasers line for flow cytometry. To help you choose the right fluorochromes for your experiments, we have developed a fluorochrome atlas that allows you to view and compare dyes with attributes such as excitation/emission spectra, filter recommendations and relative resolution information.
Find details of your fluorochromes from the dye spectra table below.
| Excitation Laser Line | Fluorochrome | Ex-max (nm) | Em-max (nm) | Relative Brightness |
|---|---|---|---|---|
| UV 355 nm |
BD Horizon™ BUV395 | 348 | 395 | |
| BD Horizon™ BUV496 | 350 | 496 | ||
| BD Horizon™ BUV563 | 350 | 564 | ||
| BD Horizon™ BUV615 | 350 | 615 | ||
| BD Horizon™ BUV661 | 350 | 660 | ||
| BD Horizon™ BUV737 | 350 | 735 | ||
| BD Horizon™ BUV805 | 351 | 803 | ||
| Violet 405 nm |
BD Horizon™ BV421 | 407 | 423 | |
| BD Horizon™ V450 | 405 | 450 | ||
| Pacific Blue™ | 404 | 455 | ||
| BD Horizon™ BV480 | 440 | 479 | ||
| BD Horizon™ V500 | 415 | 499 | ||
| BD Horizon™ BV510 | 405 | 512 | ||
| BD Horizon™ BV570*** | 407 | 573 | ||
| BD Horizon™ BV605 | 407 | 605 | ||
| BD Horizon™ BV650 | 406 | 649 | ||
| BD Horizon™ BV711 | 407 | 713 | ||
| BD Horizon™ BV75O | 409 | 754 | ||
| BD Horizon™ BV786 | 407 | 786 | ||
| Blue 488 nm |
BD Horizon™ BB515 | 490 | 515 | |
| Alexa Fluor™ 488 | 494 | 517 | ||
| FITC | 494 | 518 | ||
| BD Horizon™ RB545 | 496 | 545 | ||
| BD Horizon™ RB613 | 492 | 613 | ||
| BD Horizon™ RB670 | 492 | 670 | ||
| PerCP** | 481 | 675 | ||
| PerCP-Cy5.5** | 482 | 676 | ||
| BD Horizon™ BB700 | 476 | 695 | ||
| BD Horizon™ RB705 | 498 | 707 | ||
| BD Horizon™ RB744 | 498 | 746 | ||
| BD Horizon™ RB780 | 498 | 781 | ||
| Yellow-Green 561 nm |
PE* | 566 | 576 | |
| BD Horizon™ RY586 | 564 | 586 | ||
| BD Horizon™ RY610 | 557 | 610 | ||
| BD Horizon™ PE-CF594* | 566 | 615 | ||
| PE-Cy5* | 566 | 670 | ||
| BD Horizon™ RY703 | 557 | 703 | ||
| BD Horizon™ RY775 | 557 | 775 | ||
| PE-Cy7* | 566 | 781 | ||
| Red 640 nm |
APC | 651 | 660 | |
| Alexa Fluor™ 647 | 653 | 669 | ||
| BD Horizon™ APC-R700 | 651 | 706 | ||
| BD Horizon™ Red 718 | 695 | 718 | ||
| Alexa Fluor™ 700 | 697 | 719 | ||
| APC-Cy7 | 651 | 779 | ||
| BD® APC H7 | 659 | 782 |
BUV, BD Horizon Brilliant™ UV; BV, BD Horizon Brilliant™ Violet; V, BD Horizon™ Violet; BB, BD Horizon Brilliant™ Blue; BYG, BD Horizon Brilliant™ Yellow-Green
*Excited by 488-nm, 532-nm and 561-nm lasers.
**Excited by 488-nm and 561-nm lasers.
***Available as custom.
For additional fluorochrome selection support, launch the BD® Spectrum Viewer Tool.
Ultraviolet Laser
BD Horizon™ BUV395
BD Horizon Brilliant™ Ultraviolet 395 (BUV395) (Ex Max 348 nm/Em Max 395 nm) is a UV-excitable dye that has been developed exclusively by BD Biosciences for instruments equipped with a 355 nm UV laser. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
Filter: 379/28
Relative brightness: Moderate*
BD Horizon™ BUV496
BD Horizon Brilliant™ Ultraviolet 496 (BUV496) (Ex Max 350 nm/Em Max 496 nm) is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission maximum at 496 nm. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon™ V500 or BV510 (for example, 525/40 nm filter). BUV496 has been exclusively developed by BD Biosciences for instruments equipped with a 355 nm UV laser.
Filter: 515/30
Relative brightness: Moderate*
FLUOROCHROMES
BD Horizon™ BUV563
BD Horizon Brilliant™ Ultraviolet 563 (BUV563) (Ex Max 350 nm/Em Max 564 nm) is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an Em Max at 564 nm. Due to the excitation of the acceptor dye by other laser lines, there may be spillover into channels detecting PE (e.g., 575/26 nm filter) and PE-CF594 (e.g., 610/20 nm filter). BUV563 has been exclusively developed by BD Biosciences for instruments equipped with a 355 nm UV laser.
Filter: 560/40 or 585/15
Relative brightness: Bright*
Sample data:
BD Horizon™ BUV615
BD Horizon Brilliant™ Ultraviolet 615 (BUV615) (Ex Max 350 nm/Em Max 615 nm) is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BD Horizon™ BUV395 donor with an excitation maximum (Ex Max) of 350 nm and an acceptor dye with an emission maximum (Em Max) at 615 nm. BD Horizon™ BUV615, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 615 nm (e.g., 610/20 bandpass filter). The acceptor dye can be excited by the blue (488 nm) and yellow-green (561 nm) lasers resulting in cross-laser excitation and fluorescence spillover.
Filter: 610/20
Relative brightness: Bright*
Sample data:
FLUOROCHROMES
BD Horizon™ BUV661
BD Horizon Brilliant™ Ultraviolet 661 (BUV661) (Ex Max 350 nm/Em Max 660 nm) is a tandem dye that combines BUV395 and an acceptor dye with an emission maximum at 660 nm. As part of the BD Horizon Brilliant™ Ultraviolet family, BUV661 provides an additional option for multicolor panels utilizing UV excitable dyes. The acceptor dye can be excited by the red (628–640 nm) laser resulting in cross-laser excitation and fluorescence spillover.
Filter: 670/25
Relative brightness: Bright*
Sample data:
Lysed whole blood stained with Hu CD3 or Hu CD11c BUV661 and appropriate isotype control.
BD Horizon™ BUV737
BD Horizon Brilliant™ Ultraviolet 737 (BUV737) (Ex Max 348 nm/Em Max 737 nm) is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an emission maximum at 735 nm. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting dyes with properties like Alexa Fluor™ 700 (for example, 712/20 nm filter). BUV737 has been exclusively developed by BD Biosciences for instruments equipped with a 355 nm UV laser.
Filter: 740/35
Relative brightness: Bright*
Sample data:
BD Horizon™ BUV805
BD Horizon Brilliant™ Ultraviolet 805 (BUV805) (Ex Max 351 nm/Em Max 803 nm) is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an emission maximum at 803 nm. BUV805 has been exclusively developed by BD Biosciences for instruments equipped with a 355 nm UV laser.
Filter: 820/60 or 780/60
Relative brightness: Dim*
Sample data:
*Relative resolution for each dye was determined based on the average stain index measured across multiple specificities and instruments. Relative resolution and absolute stain index values may change depending on instrument platform and instrument configuration, including lasers, laser power and filters.
Violet Laser
FLUOROCHROMES
BD Horizon™ BV421
BD Horizon Brilliant Violet™ 421 (BV421) (Ex Max 407 nm/Em Max 423 nm) is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BV421, Pacific Blue™ and BD Horizon™ V450 cannot be used simultaneously.
Filter: 431/28 or 450/50
Relative brightness: Very Bright*
Sample data:
Comparison data from two regulatory T cell panels
T cell subset resolved: CD8 memory and effector T cells
T cell subset resolved: CD4 memory and effector T cells
Example data from a 7-color TNK panel
Example of BV421 used in an intracellular staining protocol
Example of BV421 staining using various protocols
FLUOROCHROMES
BD Horizon™ V450
BD Horizon™ Violet 450 (V450) (Ex Max 405 nm/Em Max 450 nm) is part of the BD Horizon™ Violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405 nm and an emission maximum (Em Max) at 450 nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/50 nm bandpass filter). The dye can be excited by the UV (355 nm) laser resulting in cross-laser excitation and spillover.
Filter: 450/50
Relative brightness: Dim*
BD Horizon™ BV480
BD Horizon Brilliant Violet™ 480 (BV480) (Ex Max 440 nm/Em Max 479 nm) is part of the BD Horizon Brilliant Violet™ family of dyes. This Sirigen base dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 440 nm and an emission maximum (Em Max) at 479 nm. BD Horizon™ BV480, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 480 nm (eg, a 525/50 bandpass filter).
Filter: 525/50
Relative brightness: Bright*
Sample data:
BD Horizon™ V500
BD Horizon™ Violet 500 (V500) (Ex Max 415 nm/Em Max 499 nm) is part of the BD Horizon™ Violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 415 nm and an emission maximum (Em Max) at 499 nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 500 nm (e.g., a 525/50 nm bandpass filter).
Filter: 525/50
Relative brightness: Dim*
Sample Data:
FLUOROCHROMES
BD Horizon™ BV510
BD Horizon Brilliant Violet™ 510 (BV510) (Ex Max 327 nm / 405 nm/Em Max 512 nm) is part of the BD Horizon Brilliant Violet™ family of dyes. This Sirigen base dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 327 nm / 405 nm and an emission maximum (Em Max) at 512 nm. BD Horizon™ BV510, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 510 nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355 nm) laser resulting in cross-laser excitation and spillover. BD Horizon™ BV510 can be detected in the BD Horizon™ V500 (525/50 nm) filter set.
Filter: 525/50
Relative brightness: Moderate*
Sample data:
FLUOROCHROMES
BD Horizon™ BV605
BD Horizon Brilliant Violet™ 605 (BV605) (Ex Max 407 nm/Em Max 605 nm) is a tandem fluorochrome that combines BD Horizon™ BV421 and an acceptor dye. These conjugates are very bright, exhibiting brightness similar to equivalent PE conjugates. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover of the BV605 signal into the PE and BD Horizon™ PE-CF594 detectors off the green or yellow-green lasers.
Filter: 610/20
Relative brightness: Bright*
Sample data:
FLUOROCHROMES
BD Horizon™ BV650
BD Horizon Brilliant Violet™ 650 (BV650) (Ex Max 406 nm/Em Max 649 nm) is a tandem fluorochrome of BD Horizon™ BV421 and an acceptor dye with an Em Max at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BD Horizon™ BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Filter: 660/20
Relative brightness: Very Bright*
FLUOROCHROMES
BD Horizon™ BV711
BD Horizon Brilliant Violet™ 711 (BV711) (Ex Max 407 nm/Em Max 713 nm) is a tandem fluorochrome of BD Horizon™ BV421 and an acceptor dye with an Em Max at 713 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors.
Filter: 712/20
Relative brightness: Very Bright*
Sample data:
