Power What Matters to You in Immunology Research
Join us for an exclusive educational event designed to help you streamline panel design, extract deeper insights from every sample, and harness the full power of spectral flow cytometry. Through expert insights and real-world applications, you’ll learn how to do more with less—capturing richer, more meaningful data with greater efficiency.
Where
AC Hotel San Francisco Airport/Oyster Point Waterfront
1333 Veterans Blvd.
Room Name: Liberty Ballroom
South San Francisco, CA 94080
Parking: Complimentary parking: Self-parking, around the hotel and there’s also a parking garage 2 buildings away.
Agenda
| 9:30am - 10:00am | Registration |
| 10:00-am 10:45am | Identify & Design: The Power of Choice
Discover how to select the best fluorochromes for your experiments while understanding their impact on panel performance. This module combines insights into key attributes such as brightness, spillover, stability, and background, with a structured approach for choosing reagents that aim to deliver optimal resolution of cell populations in both small and large panels.
In this module, we’ll cover:
- Fluorochrome properties that may impact panel performance, with examples.
- How to use brightness and spillover when selecting fluorochromes.
- An approach compatible with panels for conventional and spectral flow cytometry.
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| 10:45am -11:30am | Discover & Reveal: The Power of Dimensions
Explore how spectral flow cytometry combined with spatial information at the single-cell level expands the possibilities for biological insights with sub-cellular resolution. This module integrates the principles of fluorochrome selection for well-performing panels with the added dimensions from signal-derived imaging parameters to uncover hidden biology.
In this module, we’ll cover:
- How the future has changed with real-time imaging, spectral flow cytometry.
- Revealing what was missed with added dimensions from imaging parameters.
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| 11:30am-12:15pm | Networking Lunch |
| 12:15pm -1pm | Breaking Barriers with BD FACSDiscover A8: Combining Throughput, Imaging and Spectral Cytometry.
Learn how imaging cytometry can be combined with spectral cytometry to unlock biological insights. In this module we explore how CellView imaging technology can be combined with Spectral FX in several high throughput exploratory applications. We will discuss panel design considerations, experimental strategies and analysis strategies to leverage these technologies at scale.
In this module we will cover:
- High throughput imaging and spectral applications
- Spectral and Imaging fluorochrome selection
- Strategies for analyzing large imaging and spectral data sets
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| 1:00pm -2:00pm | Beyond Manual Gating: Data Science Workflows for High Dimensional Analysis using FlowJo™ Software and Data Visualization Tools
Discover how to analyze data coming from spectral instruments using FlowJo™ Software algorithms. In this module we will explore the how to perform higher-dimension analysis workflows using native and plugins algorithms available in FlowJo v10. Additionally, we will review how to incorporate imaging generated by the FACSDiscover family to this complex data analysis.
In this module we will cover:
- High parameter analysis in v10 (tSNE, UMAP, XShift, FlowSOM)
- BD CellView Lens plugin
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| 2:00pm -3:00pm | Q&A / Networking/ Ice Cream Social |
