BD Horizon Next Global Educational Tour

Identify & Design: The Power of Choice

Length: 60 minutes + Q&A

Discover how to select the best fluorochromes for your experiments while understanding their impact on panel performance. This module combines insights into key attributes such as brightness, spillover, stability, and background, with a structured approach for choosing reagents that aim to deliver optimal resolution of cell populations in both small and large panels.

In this module, we’ll cover:

• Fluorochrome properties that may impact panel performance, with examples.
• How to use brightness and spillover when selecting fluorochromes.
• An approach compatible with panels for conventional and spectral flow cytometry.

Discover & Reveal: The Power of Dimensions

Length: 60 minutes + Q&A

Explore how spectral flow cytometry combined with spatial information at the single-cell level expands the possibilities for biological insights with sub-cellular resolution. This module integrates the principles of fluorochrome selection for well-performing panels with the added dimensions from signal-derived imaging parameters to uncover hidden biology.

In this module, we’ll cover:

• How the future has changed with real-time imaging, spectral flow cytometry.
• Revealing what was missed with added dimensions from imaging parameters.
• An example of a T-bet translocation assay using 14 colors with imaging and spectral flow cytometry and 9 multiplexed samples (fluorescent cell barcoding).