Advances in Genome Biology and Technology
Join us at AGBT™ 2023 to get a sneak peek at new single-cell multiomics products and protocols from BD Biosciences. Get an opportunity to interact with top BD scientists and go hands-on with products in our dedicated hospitality suite.
Schedule a tour of our solutions with a specialist, space is limited!
| Early 1970s | Only two fluorescent dyes were available – Fluorescein and rhodamine. |
| Early 1980s | Phycobiliproteins like phycoerythrin (PE) and allophycocyanin (APC), extracted from cyanobacteria and algae, were developed by Vernon Oi, Alex Glazer and Lubert Stryer. |
| Late 1980s | The ability of PE to absorb and transfer energy to other fluorescent molecules was used to create tandem dyes (eg: PE-Texas Red, PE-Cy5, PE-Cy5.5, PE-Cy7). |
| 1990s | APC-based tandem dyes synthesized. Alexa™ dyes, which are a large spectrally-resolved series of small organic dyes, became available. 8 - 11 colour polychromatic flow cytometry became possible. |
| Early 2000s | Introduction of fluorescent, semiconductor nanocrystals (quantum dots or Qdots™) enabled 18-colour flow-cytometry. |
| 2011 and later | BD Horizon™ Brilliant violet and ultraviolet dyes have become available, which are developed using organic polymers. These dyes and their tandems provide additional bright options at a variety of wave¬lengths and are more suitable for immunophenotyping than quantum dots overcoming the main issues Qdots™ were causing. |
Advances in Genome Biology and Technology
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