Standardization has become an essential requirement in flow cytometry analysis

In this publication, a highly standardized protocol – based on LyotubeTM reagents and application settings – has been applied to a multicentre study for the evaluation of Circulating Endothelial Cells (CECs).

Under physiological conditions, CECs are among the rarest populations of cells present in peripheral blood. They are considered potential biomarkers in many pathological settings that have an impact on vessel integrity and physiology, as well as in potentially vessel-damaging pharmacological treatments like chemotherapy.

Here, CEC physiological ranges and biological variability have been described in a cohort of 269 healthy volunteers, offering a valuable starting point for more robust clinical studies.

Read the Scientific Report publication, "A standardized flow cytometrynetwork study for the assessmentof circulating endothelial cellphysiological ranges"