The BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry.  It can be used with mouse and human cells during cell staining with cyanine-containing and cyanine-like tandem fluorochrome-conjugated antibodies.

Integrating the BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer into standard flow cytometry staining protocols is simple – add the reagent to either the cell suspension or antibody cocktail. The BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer is compatible with commonly used buffers such as the BD Horizon^™ Brilliant Stain Buffer, BD Pharmingen^™ Mouse Fc Block^™ and BD Pharmingen^™ Human BD Fc Block^™

The BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer should be used when staining cells with cyanine-containing and cyanine-like tandem fluorochromes such as PE-Cy5, PE-Cy7, APC-Cy7, APC-H7 and PE-CF594. These fluorochromes can contribute to varying levels of background staining on monocytes and lymphocytes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces non-specific interactions of the fluorochrome with leucocytes, revealing true expression of your target markers.