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Multicolor Flow Cytometry Panel Design

 

Multicolor flow cytometry enables the simultaneous analysis of multiple markers at the single-cell level. By using fluorochrome conjugated antibodies that target specific cellular markers, researchers can analyze various cell characteristics like surface antigens and intracellular proteins. The design of a flow cytometry panel can be challenging and requires an understanding of several factors that can influence panel performance such as the fluorochromes and the instrument being used.

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Optimizing Your Flow Cytometry Protocol

Flow Cytometry can be a complex process, requiring specialized expertise. Particularly, developing an optimized panel design for protein analysis requires careful consideration of factors such as marker identification and fluorochrome selection. At BD Biosciences, we are committed to guiding you through every step of this intricate process, ensuring that you achieve reliable and meaningful results.

 

Doing spectral? Check out our spectral flow cytometry resources.

 

When building assays, we are presented with hundreds of fluorochrome options and their different properties can make selection feel overwhelming. Check out our Fluorochrome Performance Chart with a handy cheat-sheet to help you prioritise clean fluorochromes and simplify your panel design.

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"With the advice of the BD support team and with some minor tweaks while titrating and testing the panels, we were able to set up and validate those panels in a short timeframe and we were very happy with the results. The studies in which the panels were used were published in 2022 (PMID 35838348) and 2024 (PMID 38548324)."

 

Dr. Carolien van de Sandt

Sanquin Research

Landsteiner Laboratory

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Getting started with a simple panel design guide

Getting started with a simple panel design guide

A step-by-step guide focusing on important elements to consider when conducting single-cell analysis with flow cytometry.

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Equipping you with all the tools that you need

Equipping you with all the tools that you need

Essential tools and resources to understand key flow cytometry principles and how to make informed reagent choices.

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Build your panel with BD scientists

Build your panel with BD scientists

One-to-one personalized support from our experienced scientists to help you maximise your experiments.

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Panel Design Video Guide
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