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These introductory modules introduce you to the principles and basics concepts of flow cytometry and BD flow cytometers
This course is designed for individuals who are new to flow cytometry or want to refresh or update their knowledge. In this course, you will learn key terms and principles while gaining a better understanding of the cytometer subsystems and how they work together. Basic data analysis and sorting principles are also introduced.
Each module begins with an introduction to the topic and ends with a short quiz to check your knowledge. You can view each module separately, or you can view all the modules for a comprehensive overview of flow cytometry.
The overview module introduces the basics of flow cytometry. After viewing this module, you will be able to answer the questions:
The optics module takes a deeper dive into how the excitation and collection optics work in a flow cytometer. After viewing this module, you will be able to answer the questions:
The fluidics module explores how a sample is introduced into the flow cytometer and is interrogated by the laser. After viewing this module, you will be able to answer the questions:
The electronics module describes how data are processed. After viewing this module, you will be able to answer the questions:
The optical measurement module explores what happens to the light scatter after a particle passes through the laser beam. After viewing this module, you will be able to answer the questions:
The data analysis module looks at how flow cytometric data are collected and analyzed to produce meaningful results. After viewing this module, you will be able to answer the questions:
The sorting module introduces the technology of physically separating mixed populations of cells or particles by their light scatter or fluorescent properties. After viewing this module, you will be able to answer the questions:
