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BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kits

Enables whole transcriptome analyses of a single cell

Overview

The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit enables you to generate highly reproducible WTA data on the BD Rhapsody™ Single-Cell Analysis System. The kit has been tested and validated to generate consistent, high-quality whole transcriptome data across different workflows and users and a wide range of cell inputs.

FEATURES

The BD Rhapsody™ WTA Amplification Kit allows you to build targeted panels based on your WTA data

The kit:

Allows you to identify the most differentially expressed genes in your samples, which can then be used to construct a targeted panel specific to your research needs
Is designed to work reliably across a wide range of cell inputs from 1,000 to 10,000 cells
Works seamlessly with the BD^® Single-Cell Multiplexing Kit and enables you to run multiple samples at once without compromising the quality of your data and to save time and money

PERFORMANCE

The BD Rhapsody™ WTA Amplification Kit generates consistent and reproducible data across multiple users and cell types

Reproducible data from the same sample processed using two different cartridges

Reproducible WTA data from the same sample processed by three different users

Consistent data across a wide range of cell inputs

APPLICATIONS

The BD Rhapsody™ WTA Amplification Kit has been used to identify major immune cell types

t-SNE plot showing the major immune cell types identified with the BD Rhapsody™ WTA Amplification Kit.

The BD Rhapsody™ WTA Amplification Kit can be used in combination with the BD^® Single-Cell Multiplexing Kit to analyze multiple samples simultaneously

Jurkat and Ramos cells labeled with four unique sample tags from the BD^® Human Single-Cell Multiplexing Kit were mixed at an equal ratio (1:1:1:1), pooled and processed using the BD Rhapsody™ Single-Cell Analysis System. Libraries were generated using the BD Rhapsody™ WTA Amplification Kit.