AbSeq Protocols

Cell Labeling

1. Single Cell Labelling with BD™ AbSeq Ab-Oligos

• This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).
• Doc ID: 214394 Rev. 1.0
• Revision date: 07/2018

1. Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD® AbSeq Ab-Oligos (1 plex to 40 plex)

• This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.
• Doc ID: 214419 Rev. 2.0
• Revision date: 02/2019

1. Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD® AbSeq Ab-Oligos (41 plex to 100 plex)

• This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.
• Doc ID: 23-22354-00
• Revision date: 05/2020

Sequencing Library Preparation

1. mRNA Targeted and BD™ AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit

• This protocol provides instructions on creating mRNA and BD™ AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
• Doc ID: 214293 Rev.3.0/23-21343-00
• Revision date: 02/2019

1. mRNA Targeted, Sample Tag, and BD™ AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit

• This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
• Doc ID: 214508 Rev.3.0/23-21335-00
• Revision date: 02/2019

1. mRNA Whole Transcriptome Analysis (WTA) and AbSeq Library Preparation Protocol

• This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers. 
• Doc ID: 23-21751-00
• Revision date: 07/2019

1. mRNA Whole Transcriptome Analysis (WTA), AbSeq, and Sample Tag Library Preparation Protocol

• This protocol provides instructions on creating single cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers. 
• Doc ID: 23-21752-00
• Revision date: 07/2019

1. VDJ CDR3 and BD AbSeq Library Preparation Protocol  (Human)

• This protocol provides instructions on creating single-cell mRNA and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells. 
• Doc ID: 23-22203-01
• Revision date: 05/2020

1. VDJ CDR3, Sample Tag, and BD AbSeq Library Preparation Protocol (Human)

• This protocol provides instructions on creating single-cell mRNA, Sample Tag, and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells for human samples. 
• Doc ID: 23-22204-01
• Revision date: 05/2020