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This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
In this technical bulletin, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD® AbSeq Ab-Oligos).
The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation.
BD® Flex Single-Cell Multiplexing Kits utilize an innovative antibody-oligo technology to provide higher sample throughput for single-cell library preparation, as well as improve detection and elimination of multiplet data from single-cell experiments.
This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen-expression profiling with BD Rhapsody™ single cell capture and downstream library preparation.
This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) for antigen expression profiling with BD Rhapsody™ single-cell capture and downstream library preparation.
This protocol provides instructions on cell labeling with dCODE Dextramer® and optional BD AbSeq Ab-Oligos.
This protocol describes use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) or BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793).
This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781).
This protocol details how to stain for intracellular antigens using BD® Intracellular AbSeq (IC-AbSeq) antibodies for profiling with the BD Rhapsody™ system.
This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
The IDP consists of 30 different specificities against major human immune markers combined in a single tube. Designed and optimized to work on the BD Rhapsody™ Single-Cell Analysis System, the IDP is tested to work seamlessly with BD Rhapsody Single-Cell RNA Assays and Multiplexing Kits. In this brochure, we also describe steps for reconstituting the lyophilized IDP and staining cells.
This protocol describes cell loading in the BD Rhapsody™ Cartridge and single cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
This protocol describes cell loading in the BD Rhapsody™ Cartridge and single cell capture with the BD Rhapsody™ Single-Cell Analysis system with the BD Rhapsody™ Scanner.
This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
This protocol enables users to use cell surface proteins to profile cells independently of mRNA. Included here are instructions for how to generate BD® AbSeq single-cell libraries using the BD Rhapsody™ Single-Cell Analysis system, or the BD Rhapsody™ Express Single-Cell Analysis system. Sequencing of libraries are completed on Illumina sequencers followed by data analysis that utilizes BD® AbSeq-based cell calling pipeline which identifies cells without mRNA information.
This protocol enables users to use cell surface proteins to profile cells independently of mRNA. Included here are instructions for how to generate BD® AbSeq single-cell libraries and Sample Tag libraries using the BD Rhapsody™ Single-Cell Analysis system, or the BD Rhapsody™ Express Single-Cell Analysis system. Sequencing of libraries are completed on Illumina sequencers followed by data analysis that utilizes a BD protein cell calling pipeline which identifies cells without mRNA information.
This protocol provides instructions on creating a single cell whole transcriptome mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell whole transcriptome mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
This protocol provides instructions on creating a single cell mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating mRNA and BD® AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD® AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
This protocol provides instructions on generating a single-cell ATAC library using the BD Rhapsody™ Single-Cell Analysis System.
This protocol provides instructions on generating a single-cell ATAC library and whole transcriptome analysis (WTA) mRNA library with cell-captured beads using the BD Rhapsody™ Single-Cell Analysis System.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and BD® AbSeq libraries.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and BD® AbSeq libraries.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before
capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and BD® AbSeq libraries.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
This protocol enables high throughput single-cell transcriptome alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, and BD® AbSeq libraries.
This protocol enables high throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, BCR, BD® AbSeq, and Sample Tag libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
This guide provides detailed instructions on how to setup and run the BD Rhapsody™ Sequence Analysis Pipeline on the Seven Bridges Genomics platform or on a local server installation.
SeqGeq was built by and for scientists, with powerful data reduction tools to help you discover, cluster, and subset populations and relationships. It also includes visualization tools—including heat mapping and easy-to-use report editors—to help you communicate your results. This site provides high-level descriptions of some of SeqGeq’s core functions.
In this technical bulletin, we show how isolated nuclei can be captured and amplified on the BD Rhapsody™ Single-Cell Analysis System.
The purpose of this protocol is to provide detailed instructions on how to use single-color flow cytometry to analyze expression of oligonucleotide-conjugated antibodies with a poly(A) tail. This protocol allows rapid analysis of the expression of protein targets in the sample of interest when labelling cells with Sample Tags from the BD® Single-Cell Multiplexing Kit or with BD® AbSeq Ab-Oligos. This protocol is used prior to cDNA Synthesis
