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      APC-R700 Mouse IgG1, κ Isotype Control
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      Consider BD HorizonTM Red 718 Reagents, bright small molecule alternative to APC-R700 with lower spread into the APC detector. More Info #
      Product Details
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      BD Horizon™
      Mouse BALB/c IgG1, κ
      Keyhole limpet hemocyanin (KLH)
      Flow cytometry, Isotype control (Routinely Tested)
      0.2 mg/ml
      3811
      AB_2869632
      Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      564974 Rev. 2
      Antibody Details
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      X40

      The X40 hybridoma was derived from the fusion of mouse Sp2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice immunized with keyhole limpet hemocyanin (KLH). The hybridoma secretes monoclonal antibody that binds specifically to KLH, an antigen not expressed on human cells or human cell lines.

      This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).

      564974 Rev. 2
      Format Details
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      APC-R700
      BD Horizon™ APC-R700 is a part of the BD APC Red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and a acceptor dye, R700, with an emission maximum (Em Max) at 706 nm. APC-R700, driven by BD innovation, is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 710 nm (eg, a 720/40 nm bandpass filter). Please ensure that your instrument’s configuration (lasers and optical filters) are appropriate for this dye. Visit our Supplies, Parts and Setup Reagents page, find the link to your instrument and on the product page, navigate down to Related Products for more information.
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      APC-R700
      Red 627-640 nm
      651 nm
      706 nm
      564974 Rev.2
      Citations & References
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      View product citations for antibody "564974" on CiteAb
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      No Citations are available for this Product

      564974 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

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