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Novel approaches to single-cell plant research: From Real-Time Imaging cell sorting to single-nuclei transcriptomics

The introduction of real-time imaging in flow cytometry and the recent advancement in single-cell multiomics have revolutionized the world of cellular and molecular biology by adding new dimensions to single-cell data analysis. Unsurprisingly, these technologies are also gaining traction in plant molecular and developmental biology to reveal the transcriptional changes across cell types in a specific tissue or organ, upon treatments, in response to biotic and abiotic stresses, or between genotypes.

 

In this webinar, Gert Van Isterdael will present an overview of the plant applications they have been developing using the BD FACSDiscover™ S8 Cell Sorter in collaboration with researchers from the VIB-UGent Center for Plant Systems Biology. The use of BD CellView™ Image technology applied to protoplasts allows scientists to make faster and more confident sorting decisions, thereby improving the quality of sort outcomes and its connected downstream processes.
 

In the second part of the webinar, Dr. Carolin Grones will address the challenges of single-cell transcriptomics applied to plant tissues while proposing a novel single-nuclei approach to deliver more robust and high-quality data, thanks to the BD Rhapsody™ Single-Cell Analysis System.

Learn How:

  • Plant molecular and developmental biology can benefit from new technologies that allow real-time imaging and single-cell multiomics of plant cells.
  • The BD FACSDiscover™ S8 Cell Sorter can sort protoplasts based on their image features and improve the quality of downstream processes.
  • The BD Rhapsody™ Single-Cell Analysis System can perform single-nuclei transcriptomics on plant tissues and deliver more reliable and comprehensive data.

Speakers

Carolin Grones, PhD

Single Cell Application Specialist

VIB-UGent Center for Plant Systems Biology and Wageningen University

Gert Van Isterdael

VIB Flow Core Gent