BD OneFlow™ ALOT

Overview

The BD OneFlow™ ALOT is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.^1* 

The BD OneFlow™ ALOT is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood.

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ LST, BD OneFlow™ B-CLPD T1, BD OneFlow™ PCST, BD OneFlow™ PCD.

FEATURES

The BD OneFlow™ ALOT:

Is a pre-configured single-use, 8-color reagent
Comprises 2 ready-to-use tubes, one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube)
Can guide the need for further analysis as a screening tube in combination with panel(s) specifically designed for the classification of different forms of B cell, T cell and myeloid acute leukemias
Is available in 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes)

Allows for easy visual identification as dark red color-coded boxes, pouches and tubes

The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.²

For BD FACSLyric™ Flow Cytometer users:

BD^® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
BD^® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
BD^® CS&T Beads and BD^® FC Beads kits necessary for instrument setup and compensation can be used for other CE-IVD assays on the BD FACSLyric™ Flow Cytometer

For BD FACSCanto™ II Flow Cytometer users:

BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs³
Specifically designed BD^® FC Beads eliminate the need for using single-vial reagents and cells for compensation

The BD OneFlow™ ALOT reagent composition

Antibody	Fluorochrome	Clone	Tube	Target Populations
MP0	FITC	MP0-7	C	Myeloid lineage marker
CD79A	PE	HM57	C	B-lineage marker
CD34	PerCP-Cy5.5	8G12	S	Backbone marker (BCP-ALL and AML Panels). Identification of immature cells.
CD19	PE-Cy7	SJ25-C1	S	Backbone marker (BCP-ALL Panel). B-lineage marker
CD7	APC	M-T701	S	T-lineage marker
CD3	APC-H7	SK7	S	Backbone marker (T-ALL panel).
CD3	BD Horizon™ V450	UCHT-1	C	Backbone marker (T-ALL panel). Maturity marker for T-cells.
CD45	BD Horizon™ V500-C	2D1	S	Backbone marker. Identification of immature cells.

The EuroFlow antibody panel article¹* has a full description of the utility of the antibodies chosen for the BD OneFlow™ ALOT.

APPLICATIONS

BD OneFlow™ ALOT Lab Report (normal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of a normal peripheral blood specimen stained with the 8-color 8-antibody BD OneFlow™ ALOT single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of aberrant immature lymphoid and non-lymphoid populations.

BD OneFlow™ ALOT Lab Report (abnormal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ ALOT single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of aberrant immature lymphoid and non-lymphoid populations.

BD OneFlow™ Reagents can be run on both the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers with equivalent results.

Supporting Evidence

“An unprecedented orientation efficiency of 98.3% for non-ambiguous lineage cases was shown for the [EuroFlow] ALOT combination with a series of 483 newly diagnosed acute leukemia cases, tested prospectively at different centers.”¹*

Results from the clinical trial showed that the BD OneFlow™ ALOT gave an overall agreement of 100% (93 of 93) in orienting patients into lymphoid (44 of 44 concordant) and non-lymphoid (49 of 49 concordant) lineages when compared with the EuroFlow system. Furthermore, interpretation of clinical trial results using the BD OneFlow™ Solution was highly concordant to diagnostic truth derived from a composite of patient history and lab findings.

RESOURCES

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Instructions for Use

BD OneFlow™ Setup Beads (BD FACSCanto™ II Flow Cytometer)

BD^® FC Beads 8-Color Kit for BD OneFlow™ Assays (BD FACSCanto™ II Flow Cytometer)

Tools

BD Antibodies in EuroFlow™ Multicolor Panels

EuroFlow™ Multicolor Panels

RELATED RESOURCES

Application Note

Standardized Flow Cytometry System to Aid in the Diagnosis of Chronic Lymphocytic Leukemia

References

van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122