BD OneFlow™ PCST

Overview

The BD OneFlow™ PCST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.^1* 

The BD OneFlow™ PCST is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow.

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ LST, BD OneFlow™ B-CLPD T1, BD OneFlow™ ALOT, and BD OneFlow™ PCD.

FEATURES

The BD OneFlow™ PCST:

Is a preconfigured single-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube)
Is available in 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes)
Allows for easy visual identification with dark green color-coded boxes, pouches and tubes

The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.²

For BD FACSLyric™ Flow Cytometer users:

BD^® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
BD^® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
BD^® CS&T Beads and BD^®FC Beads kits necessary for instrument setup and compensation can be used for other IVD assays on the BD FACSLyric™ Flow Cytometer

For BD FACSCanto™ II Flow Cytometer users:

BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs³
Specifically designed BD® FC Beads eliminate the need for using single-vial reagents and cells for compensation

The BD OneFlow™ PCST reagent composition

Antibody	Fluorochrome	Clone	Tube	Target Populations
CD38	FITC	HB7	S	Backbone markers. Identification of plasma cells
CD56	PE	MY31	S	Identification of aberrant plasma cells
β2-Microglobulin	PerCP-Cy 5.5	TU99	S	Identification of aberrant plasma cells
CD19	PE-CY 7	SJ25-C1	S	Backbone markers. Identification of plasma cells. Identify and characterize mature B cells
Anti-Kappa	APC	TB28-2	C	Plasma cells clonality
Anti-Lambda	APC-H7	1-155-2	C	Plasma cells clonality
CD45	BD Horizon™ V450	2D1	S	Backbone markers. Identification of plasma cells
CD138	BD Horizon™ V500-C	MI15	S	Backbone marker. Identification of plasma cells

The EuroFlow antibody panel article1* has a full description of the utility of the antibodies chosen for the BD OneFlow™ PCST.

APPLICATIONS

BD OneFlow™ PCST Lab Report (normal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of a normal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.

BD OneFlow™ PCST Lab Report (abnormal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.

BD OneFlow™ Reagents can be run on both the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers with equivalent results.

RESOURCES

Tools

BD Antibodies in EuroFlow™ Multicolor Panels

EuroFlow™ Multicolor Panels

Related Resources

Application Note

Standardized Flow Cytometry System to Aid in the Diagnosis of Chronic Lymphocytic Leukemia

References

van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122