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The BD OneFlow™ B-CLPD T1 is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1*
The BD OneFlow™ B-CLPD T1 is intended for flow cytometric immunophenotyping of B cells in peripheral blood and bone marrow.
Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ LST, BD OneFlow™ ALOT, BD OneFlow™ PCST, and BD OneFlow™ PCD.
| Antibody | Fluorochrome | Clone | Target Populations |
|---|---|---|---|
| CD23 | FITC | EBVCS-5 | Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases |
CD10 | PE | MEM-78 | Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases |
| CD79b | PerCP-Cy 5.5 | SN8 | Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases |
| CD19 | PE-CY 7 | SJ25-C1 | Backbone marker. In common with BD OneFlow™ LST |
| CD200 | APC | MRC 0X-104 | Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases |
| CD43 | APC-H7 | 1G10 | Contributes to the classification of CLL or other B-cell chronic lymphoproliferative diseases |
| CD20 | BD Horizon™ V450 | L27 | Backbone marker. In common with BD OneFlow™ LST |
| CD45 | BD Horizon™ V500-C | 2D1 | Backbone marker. In common with BD OneFlow™ LST |
The EuroFlow antibody panel article1* has a full description of the utility of the antibodies chosen for the BD OneFlow™ B-CLPD T1.
APPLICATIONS
Laboratory report of a normal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ B-CLPD T1 single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4.
Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ B-CLPD T1 single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4.
