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Extracellular vesicles (EVs) are membranous vesicles that carry tremendous potential as biomarkers and therapeutic targets. Secreted by many cell types, these nanosized particles package diverse biological cargo and play a crucial part in mediating intercellular communication. However, because these intriguing messengers are so small and heterogeneous, they are also difficult to characterize.

 

Flow cytometry is a common technique for EV analysis1 and is widely used for EV characterization,2,3,4 but there have been several challenges associated with this method. Characterization of EVs using flow cytometry has been challenging owing to their size (30–1,000 nm),1 which is below the detection limit of regular flow cytometers (10–100 µm), and other factors such as the composition of EVs and differences in their concentrations. But innovation and standardization in flow cytometry holds promise for improved methods for their measurement.

 

BD Biosciences offers instrumentation and resources to tackle some of these challenges. EV researchers have utilized these new advances in innovative ways to resolve several issues that could not be previously addressed.

 

Read the blog entitled, “Using the latest advances in flow cytometry to tackle challenges in extracellular vesicle characterization”.

Watch the webinar organized by BD Biosciences in collaboration with Nature on the latest developments in extracellular vesicle analysis using flow cytometry with EV researchers Dr. Jonni Moore of Penn Cytomics and Cell Sorting Resource Laboratory, Dr. Terry K. Morgan of Oregon Health & Science University and Dr. Edwin van der Pol of Amsterdam University Medical Centers.

 

In this webinar, Dr. Edwin van der Pol demonstrates how he used flow cytometry to measure concentrations of EVs that were <100 nm in a reproducible and scalable way, while Dr. Moore and Dr. Morgan discuss mining of EV information from liquid biopsy samples and the detection of gestational phenotype differences using cell-specific EVs, respectively.

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      This video shows how researchers are using BD products to explore human health and potentially produce future improved treatments and diagnostics.

      References

       

      1. Gul B, Syed F, Khan S, Iqbal A, Ahmad I. Characterization of extracellular vesicles by flow cytometry: Challenges and promises. Micron. 2022;161:103341. doi: 10.1016/j.micron.2022.103341

      2. Irmer B, Efing J, Reitnauer LE, et al. Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression. Cell Commun Signal. 2023 ;21(1):171. doi: 10.1186/s12964-023-01186-1

      3. Mandelbaum N, Zhang L, Carasso S, et al. Extracellular vesicles of the Gram-positive gut symbiont Bifidobacterium longum induce immune-modulatory, anti-inflammatory effects. NPJ Biofilms Microbiomes. 2023;9(1):30. doi: 10.1038/s41522-023-00400-9

      4. Cohen SJ, Meyerovich G, Blank S, et al. Microbiota transfer following liver surgery involves microbial extracellular vesicle migration that affects liver immunity. Hepatol Commun. 2023;7(6):e0164. doi: 10.1097/HC9.0000000000000164.
      Workflow for EV Analysis
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      BD Solutions for EV Research
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      For Research Use Only. Not for use in diagnostic or therapeutic procedures.