Spectral Cytometry Success: From Design Input to Data Output

The capabilities of flow cytometry instruments in terms of available lasers, detectors and number of measurable parameters have expanded tremendously over the years. Concomitant with that, there is an exponential increase in the number of fluorochromes available for flow cytometry, extending the limits of possibilities for new discoveries.

Additionally, spectral flow cytometry collects the full spectrum, which increases the flexibility and the capability of spectral flow by enabling autofluorescence profiling and extraction, detection of more fluorochromes per laser line and increased panel size. Join our three-part series to gain a deeper understanding of spectral flow cytometry, learn how to apply these fundamentals for next-gen spectral cytometry, and see how one scientist used a 40-color spectral panel for deep immunophenotyping.

October 3, 2023 – Spectral Flow Fundamentals

Abstract:

Do you want to transition over to spectral flow cytometry? Or maybe you’re already doing spectral flow cytometry and want to improve the quality of your spectral data? Gain a deeper understanding of spectral flow cytometry by going back to fundamentals. Join Peter Mage for an engineer’s perspective on the science of spectral flow cytometry, as he explains how the concepts at the heart of spectral flow cytometry technology can inform your experiment design

October 18, 2023 – Deep Immunophenotyping of Murine Lymphoid Tissues Using a 40-Color Spectral Flow Cytometry Panel [OMIP-0XX]

Abstract:

Join us for a scientific talk by Aris Kare that will dive into their laboratory’s new publication [OMIP-0XX] covering a 40-color flow cytometry spectral experiment followed by an open Q&A and panel discussion to address key ingredients in successful spectral panel design and role of fluorochromes.

High-dimensional immunoprofiling is essential for studying host response to immunotherapy, infection, and disease in murine model systems. However, the difficulty of multiparameter panel design combined with a lack of existing murine tools has prevented the comprehensive study of all major leukocyte phenotypes in a single assay. To fill this niche, we present a 40-color flow cytometry panel for deep immunophenotyping in primary and secondary lymphoid tissues. Importantly, the panel solely relies on extracellular staining to preserve cells for downstream experiments and is capable of identifying functional lymphocyte and myeloid subsets. In this webinar, I will discuss how the panel was engineered, as well as special considerations in reagent optimization, spectral unmixing, autofluorescence extraction, and multi-dimensional analysis.

November 1, 2023 –  Elevating image-based sorting applications with spectral immunophenotyping

Abstract:

Real-time image feature extraction enables novel avenues of research and sort possibilities as we can classify and characterize cell populations based on spatial distribution of fluorescence. The possibilities broaden further when these imaging capabilities are coupled with next generation spectral immunophenotyping. Although spectral cytometry can seem intimidating or complex, our advanced spectral cytometry system, BD FACSDiscover™ S8 Cell Sorter with BD CellView™ Image Technology and BD SpectralFX™ Technology combines full-spectrum optics and optimized hardware design with a system-aware algorithm, next-generation quality control (QC) and guided software workflow so users can get started quickly. In this webinar, Aaron Middlebrook, Sr. Staff Scientist, R&D, BD, and Peter Mage, Associate Principal Engineer, Advanced Technology Group, BD will demonstrate the power of real-time imaging, spectral flow cytometry (RTI-SFC), share best practices in experimental setup and examples of application using this novel technology.