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Overview
The BD Rhapsody™ Sequence Analysis Pipeline is a versatile tool that offers the flexibility to run your bioinformatics analysis on either a Seven Bridges cloud-based platform or on a local installation.
The BD Rhapsody™ Sequence Analysis Pipeline:
- Provides a primary analysis of single-cell multiomics data by leveraging cutting-edge algorithms to deliver fast results and deep insights.
- Utilizes an intuitive user interface via a cloud-based platform and is easy to use, regardless of the computational expertise of the user.
- Offers the ability to choose between cloud-based or local installation options and affords maximum convenience and accessibility for single-cell multiomics data analysis.
- Provides broad compatibility of output data with downstream analysis tools such as Seurat and Scanpy.
Pipeline Overview
After sequencing, the pipeline takes input from FASTQ files, a reference (Targeted panel or WTA genome archive), an AbSeq reference (if required), and a supplemental reference (if required), using those to generate molecule counts per cell and metrics about the pipleline run.
Overview of the steps in the analysis pipeline.
Features
- Free : Upload raw data, run the pipeline and download results from the cloud for free
- Fast: Less than 3 hours to process 1 billion reads
- Simple: One consolidated pipeline for BD Rhapsody™ Whole Transcriptome Analysis Amplification Kit, BD Rhapsody™ Targeted mRNA Kits, BD Rhapsody™ TCR/BCR Next Multiomic Assays and BD Rhapsody™ ATAC-Seq Assays