BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer

The BD Pharmingen^™ MonoBlock^™Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry. It can be used with mouse and human cells during cell staining with cyanine-containing and cyanine-like tandem fluorochrome-conjugated antibodies.

Integrating the BD Pharmingen^™MonoBlock^™ Leukocyte Staining Buffer into standard flow cytometry staining protocols is simple – add the reagent to either the cell suspension or antibody cocktail. The BD Pharmingen^™MonoBlock^™ Leukocyte Staining Buffer is compatible with commonly used buffers such as the BD Horizon^™ Brilliant Stain Buffer, BD Pharmingen^™ Mouse Fc Block^™and BD Pharmingen^™ Human BD Fc Block^™

The BD Pharmingen^™ MonoBlock^™Leukocyte Staining Buffer should be used when staining cells with cyanine-containing and cyanine-like tandem fluorochromes such as PE-Cy5, PE-Cy7, APC-Cy7, APC-H7 and PE-CF594. These fluorochromes can contribute to varying levels of background staining on monocytes and lymphocytes. The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces non-specific interactions of the fluorochrome with leucocytes, revealing true expression of your target markers.

Flow cytometric analysis of myeloid and lymphoid cells with cyanine-containing tandem fluorochromes.

BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on human leukocytes, while maintaining antibody-specific staining

When staining lysed whole blood with cell surface markers (CD279, CD3, CD19) conjugated to PE-Cy7, there is background staining on monocytes. Addition of the BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer to the staining protocol reduces the nonspecific background staining derived from PE-Cy7 on monocytes but does not affect antibody-specific staining of CD279 (PD-1), CD3 and CD19 staining on lymphocytes.

BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer reduces background staining on mouse leukocytes

Background staining with cyanine-containing and cyanine-like tandem fluorochromes can also be observed in mouse leukocytes. In addition, some cell types express Fcγ receptors that can also contribute to background staining by binding non-specifically to detection antibodies. This is commonly addressed by using reagents that block Fc receptors such as BD Pharmingen^™Mouse Fc Block^™. BD Pharmingen^™ MonoBlock^™ Leukocyte Staining Buffer can be used in combination with Fc blocking reagents. Together, they help resolve true positive populations from false negatives derived from dye and Fc receptor-mediated staining.

When staining mouse bone marrow cells with PE-Cy7 antibody conjugates without blocking reagents, both lymphoid and myeloid populations show background staining. Treatment with BD Pharmingen^™ Mouse Fc Block^™ reduces some of the background staining, but non-specific binding of the fluorochrome is still present. Staining cells in the presence of both BD Pharmingen^™Mouse Fc Block^™ and the BD Pharmingen^™MonoBlock™ Leukocyte Staining Buffer shows further reduced background across three different antibody isotypes. The BD Pharmingen^™MonoBlock^™Leukocyte Staining Buffer reduces background staining on leukocytes, while preserving the staining intensity of antibody-specific binding such as CD11b.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

The BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer is formulated to block nonspecific binding with cyanine-containing and cyanine-like tandem fluorochromes to leukocyte subsets, especially monocytes and macrophages, in flow cytometry.