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RY586
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Introducing
BD Horizon RealYellow™ 586 (RY586) Reagents

BD Horizon RealYellow™ 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. They are excited primarily by the 561-nm yellow-green laser and offer:

 

  • Minimal cross-laser excitation off the 488-nm blue laser
  • Bright fluorescence to detect low-expression surface and intracellular markers 

 

Conventional flow cytometry: Just as bright as PE, but with significantly less cross-laser excitation, RY586 reagents can be used instead of PE on conventional flow cytometers for more flexible panel design.

 

Spectral flow cytometry: With a distinct spectral profile from PE, RY586 reagents can be used with PE on spectral flow cytometers to increase parameters for deep scientific insights.

 
performance1

Excellent Resolution

Generate reliable data that clearly resolve even the most challenging cell populations by leveraging bright, clean fluorochromes that minimize spillover and background.

Flexible Panel Design

Maximize panel design options for both conventional and spectral flow cytometry by using reagents that deliver minimal cross-laser excitation in hundreds of antibody specificities.

High-Dimensional Analysis

Reveal more insights from the same sample by expanding the utility of blue and yellow-green laser channels with reagents that enable the simultaneous use of more detectors.

Performance

Click through the slides below to view performance data.

Clean & Bright

 

BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation

 

BD Horizon RealYellow™ 586 Reagents exhibit minimal cross-laser excitation off the blue laser, resulting in significantly less spillover into blue detectors than PE. At the same time, they offer minimal background and high resolution comparable to PE.
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Versatile Applications

 

BD Horizon RealYellow™ 586 Reagents have versatile applications

 

RY586 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RY586 reagents can be used instead of PE on conventional flow cytometers or with PE on spectral flow cytometers equipped with the 561-nm yellow-green laser to meet your specific panel design needs.
View Available Reagents

Spectral Flow Cytometry

 

BD Horizon RealYellow™ Reagents can be used with spectral flow cytometry

 

RY586 can be used with PE on spectral flow cytometers. Since RY586 provides comparable brightness to PE, it is ideal for detecting low-expression markers. Using RY586 and PE in the same spectral flow cytometry panel enables high-dimensional analysis of more low-expression surface and intracellular markers.

Stable & Consistent

 

Performance stability of BD Horizon RealYellow™ Reagents RY586 offers stable reagent performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents as well as proven photostability when exposed to typical lab lighting or dimmed core lab lighting.
Background data RY586

RY586 made-to-stock reagents and BD OptiBuild™ On-Demand Reagents show consistent performance between each other and across multiple lots.

 

Human whole blood was stained with human CD4 (SK3) RY586, followed by lysis with BD FACS™ Lysing Solution. Mouse splenocytes were stained with mouse CD8a 53-6.7. All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.

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BD Horizon RealYellow™ Reagents offer wide compatibility

BD Horizon RealYellow™ Reagents are compatible with a variety of common fixation and permeabilization systems, including:

 
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RB780
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Performance Applications

BD Horizon RealBlue™ 780 (RB780) Reagents—the superior alternative to PE-Cy7

RB780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser to offer.

 

  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Bright fluorochrome to support the detection of low-expression surface and intracellular markers 
  • Reduced monocyte background compared to PE-Cy7

 

Download the BD Horizon RealBlue™ 780 Reagents Brochure >

 
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RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers

 

RB780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.

View available reagents >

Performance

BD Horizon™ RB780 Reagents offer minimal cross-laser excitation off the yellow-green laser and reduced background compared to PE-Cy7

RB-780 Emission Profile

Normalized emission profile of PE-Cy7 and RB780 with RB780 minimal excitation off the 561-nm yellow-green laser.

Human whole blood was stained with BD Horizon™  RB780 Human CD4 (SK3) Reagent, acquired and compensated on a BD FACSymphony™  A5 SE Cell Analyzer.

BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7

Applications

BD Horizon™ RB780 Reagents support the detection of low-expression surface and intracellular markers

RB780 reagents can resolve low-expression surface and intracellular markers and are available in a wide array of specificities. RB780 reagents can be used instead of PE-Cy7 on conventional flow cytometers or with PE-Cy7 on spectral flow cytometers to meet your specific panel design needs.
View Available Reagents >
RB780 reagents and PE-Cy7 can be used together in spectral flow cytometry
BD Horizon™ RB780 Reagents offer stable reagent performance and photostability

The BD Horizon™ RB780 Reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.
Human whole blood was stained with human CD4 (SK3) RB780, followed by lysis with BD FACS™ Lysing Solution using seven different batches of reagent (left). Mouse splenocytes were stained with mouse CD8a 53-6.7 using six different batches of reagent (right). All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.
View available reagents >

BD Horizon RealBlue™ 780 Reagents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm III BufferCompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible

 

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RB545
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Performance Applications

BD Horizon RealBlue™ 545 (RB545) Reagents

RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:

 

  • Minimal cross-laser excitation off the 561-nm yellow-green laser
  • Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

 

Download the BD Horizon RealBlue™ 545 Brochure>

 
RB545 vials
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RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

RB545 specifications

View available reagents >

Performance

BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

RB545 Normalized Emission Filter Parameters
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RB545 Spread Plots
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Applications

BD Horizon™ RB545 Reagents support the detection of high-expression surface and intracellular markers

RB545 reagents can resolve high-expression surface and intracellular markers and are available in a wide array of specificities. RB545 reagents can be used with PE and FITC on spectral flow cytometers to meet your specific panel design needs.
Surface Marker
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RB545 and FITC can be used together in spectral flow cytometry
RB545 with FITC Overlays
TBNK Aurora top plots
BD Horizon RealBlue™ 545 Reagents are a superior alternative to FITC when used together with BD Horizon Brilliant™ Blue 515 (BB515) Reagents in high-parameter spectral flow cytometry panels

RB545 reagents can help improve resolution in high-parameter spectral flow cytometry panels.
CD4 RB545-CD127 BB515

Human PBMCs were stained with two sets of reagents, moderately similar CD4 RB545 and CD127 FITC, and highly similar CD4 FITC and CD127 BB515. Co-expressed markers were selected to demonstrate impact of spectral unmixing with different levels of similarity. Resolution was impacted when reagents were highly similar, shown in bottom set of CD4 FITC and CD127 BB515. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with FlowJo™ Software.

View available reagents >
BD Horizon RealBlue™ 545 Reagents offer stable reagent performance and photostability

RB545 reagents show stable performance with lot-to-lot consistency across made-to-stock reagents and BD OptiBuild™ On-Demand Reagents. They also offer proven photostability when exposed to typical lab lighting or dimmed core lab lighting.
Human CD4 compared with Mouse CD8
Human whole blood was stained with human CD4 (SK3) RB545 reagent, followed by lysis with BD FACS™ Lysing Solution using four different batches of reagent (left). Mouse splenocytes were stained with mouse CD8a (53-6.7) using four different batches of reagent (right). All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.
View available reagents >

BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

 

BuffersResults
BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
CellBlox™ Blocking BufferCompatible
BD Cytofix™ Fixation BufferStable at least 24 hours
1% PFAStable at least 24 hours
BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
BD Phosflow™ Perm III BufferCompatible with antibody staining before and after fixation
EDTA and HeparinCompatible
BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
 
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