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      Protocols

       

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      Cell Biology

      Immunoprecipitation with Antibody: Agarose Conjugates
      Immunoprecipitation with Anti-Phosphotyrosine: Biotin Conjugates
      Immunoprecipitation with Soluble Antibodies
      Western Blotting with Rabbit Polyclonal Antibodies
      Western Blotting with Monoclonal Antibodies
      Bioimaging in 96 well plates
      Western Blotting with Biotinylated Antibodies
      Immunoprecipitation with Antibody: Agarose Conjugates
      Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
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      Flow Cytometry

      Procedure for Manual Setting of Compensation for Multicolor Flow Cytometry Cytometric Analysis
      Uses of BD Fc Block™ Reagent
      Stain Lyse Wash
      Stain Lyse No Wash
      BD PhosFlow™ Buffer Cytometry Protocols for Mouse Splenocytes or Thymocytes
      Mouse and Rat Leukocytes
      Isotope Control
      Indirect Staining Mononuclear Cells
      Immunopurification of Tyrosine Phosphorylated Proteins
      BD PhosFlow™ Buffer Cytometry Protocol for Human PBMCs
      BD PhosFlow™ Buffer Cytometry Protocol for Human Whole Blood Samples
      The Escapee Phenomenon
      Fix Perm Kits
      Protocol Direct Immunofluorscence Staining
      Cytokines FCA
      Cell Surface
      Cytokine Stimulation and 96 Well BD Phosflow™ Buffer Protocol
      TCR Stimulation and 96 Well BD Phosflow™ Buffer Protocol
      Activation Immune Cells
      BD Phosflow™ Buffer Protocol for Adherent Cells
      Preparation and Staining of Paraffin Sections
      Intracellular Flow and Phosflow FAQ
      Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes Download Icon Alt Text
      Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs Download Icon Alt Text
      Generation of Human IL-17–Producing Cells (Th17-like Cells) In Vitro Download Icon Alt Text
      Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs Download Icon Alt Text
      Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs) Download Icon Alt Text
      Preparation of Murine Splenocytes Download Icon Alt Text
      Tissue Preparation for Surface Antigen Staining Download Icon Alt Text
      Bulk Erythrocyte Lysing with Ammonium Chloride for Flow Cytometry Cytometry Immunophenotyping Download Icon Alt Text
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      Single-Cell Multiomics

      BD Rhapsody™ Targeted mRNA & AbSeq Amp Kit
      BD Rhapsody™ WTA Amplification Kit
      BD Rhapsody™ Targeted mRNA AbSeq Kit 4 Pack (sales BOM)
      BD Rhapsody™ WTA Reagent Kit – 4 pack (sales BOM)
      Hu Single Cell Sample Multiplexing Kit
      Ms Single Cell Sample Multiplexing Kit
      BD Rhapsody™ Immune Response Panel HS
      BD Rhapsody™ T cell Expression Panel HS
      BD Rhapsody™ Onco-BC Panel HS
      BD Rhapsody™ Immune Response Panel Mm
      BD Rhapsody™ Panel Supplement
      BD Rhapsody™ Custom Panel
      BD® AbSeq Ab-Oligos (all SKU's)
      VDJ CDR3 Protocols
      BD Rhapsody™ Cartridge Reagent Kit Download Icon Alt Text
      BD Rhapsody™ Cartridge Kit Download Icon Alt Text
      BD Rhapsody™ cDNA Kit Download Icon Alt Text
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      Stem Cell

      Cell Surface Staining of Stem Cell Lines and Other Adherent Cells for Flow Cytometry Cytometry Download Icon Alt Text
      Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs Download Icon Alt Text
      Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs) Download Icon Alt Text
      Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs Download Icon Alt Text
      Preparation of Murine Splenocytes Download Icon Alt Text
      Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes Download Icon Alt Text
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