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Overview

Flow cytometry is widely used for disease-specific assessments and immunophenotyping of immune cell populations. This evaluation helps in diagnosis of immune deficiencies. Determining lymphocyte subsets can generate more insights than a complete blood count (CBC). 

Learn more about immune deficiencies
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BD Multitest™ 6-Color TBNK Reagent with optional BD Trucount™ Tubes is a six-color direct immunofluorescence reagent for use in identifying and determining the percentages and absolute counts of T, B and natural killer (NK) cells, as well as the CD4+ and CD8+ subpopulations of T cells in peripheral blood on a BD flow cytometer.

Why BD Multitest™ 6-Color
TBNK Kit?

  • Lymphocyte enumeration of mature T, B and NK cells in a single tube
  • Option of single platform absolute counts with BD Trucount™ Tubes
  • Automated instrument setup, QC, acquisition and analysis on the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers
  • Complete automated workflow from sample to answer using the BD FACSLyric™ Flow Cytometer in combination with the BD FACSDuet™ Sample Preparation System, minimizing manual handling of potentially infectious samples
  • Fully integrated automated sample preparation with the BD FACDuet™ Sample Preparation System
  • Automated sample acquisition with the BD FACS™ Universal Loader for the BD FACSLyric™ Flow Cytometer and the BD FACS™ Loader for the BD FACSCanto™ II Flow Cytometry System.
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Features

The BD Multitest™ 6-Color TBNK Kit Simplifies Your Workflow and Increases Productivity

  • An easy-to-use Solution
    With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. The reagent includes a cocktail of multiple fluorescently labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining.

  • Accurate cell counts with BD Trucount™ Tubes
    BD Trucount™ Tubes help ensure more accurate cell counts. The tubes contain a lyophilized pellet of fluorescent beads that serves as an internal standard. The BD FACSuite™ Clinical Application and BD FACSCanto™ Clinical Software (v2.0 or later) automatically calculate lymphocyte absolute counts and subset percentages.

  • Improve safety, reduce hands-on time
    The BD Multitest™ 6-Color TBNK Reagent employs a no-wash formula that reduces tube handling. Processing fewer tubes in less time may decrease potential exposure to biohazardous material, improving safety for laboratory personnel.

  • Increase productivity
    Together with the BD FACSLyric™ Flow Cytometer and BD FACSuite™ Software, the universal loader option and the BD FACSDuet™ Sample Preparation System, or the BD FACSCanto™ Flow Cytometer and BD FACSCanto™ Clinical Software, the loader option, and the BD FACS™ Sample Prep Assistant (SPA), the BD Multitest™ 6-Color TBNK Reagent provides high performance for lymphocyte enumeration to best suit your routine immunophenotyping needs.

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Applications

The BD Multitest™ 6-Color TBNK Reagent with BD Trucount™ Tubes for accurate and reliable enumeration of T, B and NK cells

A single tube, single platform IVD assay utilizing BD Trucount™ Tube technology

BD FACSLyric™ Flow Cytometer

A hematologically normal adult sample stained with BD Multitest™ 6-Color TBNK Reagents in a BD Trucount™ Tube was acquired on a BD FACSLyric™ Flow Cytometer. See figure for representative data from a sample acquired using BD Trucount™ Tubes. See the BD FACSLyric™ Clinical Reference System, which provides information on gating and troubleshooting.
TBNK-FACSLyric-Report
TBNK-FACSLyric-Report

6-Color TBNK Lab Report showing data collected with BD Trucount™ Tubes

BD FACSCanto™ Flow Cytometer

A hematologically normal adult sample stained with BD Multitest™ 6-Color TBNK Reagent in a BD Trucount™ Tube was acquired on a BD FACSCanto™ Flow Cytometer. See figure for representative data from a sample acquired using BD Trucount™ Tubes. See the BD FACSCanto™ Flow Cytometer IFU, which provides information on gating and troubleshooting.
TBNK-FACSCanto-Report
TBNK-FACSCanto-Report

BD FACSCanto™ Flow Cytometry System laboratory report showing data collected with BD Trucount™ Tubes.

References

Omana-Zapata I, Mutschmann C, Schmitz J, Gibson S, Judge K, Aruda Indig M, Lu B, Taufman D, Sanfilippo AM, Shallenberger W, Graminske S, McLean R, Hsen RI, d'Empaire N, Dean K, O'Gorman M. Accurate and reproducible enumeration of T-, B-, and NK lymphocytes using the BD FACSLyric 10-color system: A multisite clinical evaluation. PLoS One. 2019 Jan 28;14(1):e0211207. doi: 10.1371/journal.pone.0211207. PMID: 30689658; PMCID: PMC6349332.

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BD Flow Cytometers and BD FACSDuet™ Sample Preparation System are Class 1 Laser Products.

In the U.S., the BD FACSCanto™ Flow Cytometer and BD FACSCanto™ II Flow Cytometer are for In Vitro Diagnostic Use for up to six colors. Seven to ten colors are for Research Use Only.

In the U.S., the BD FACSLyric™ Flow Cytometer is for In Vitro Diagnostic Use with BD FACSuite™ Clinical Application for up to six colors.

In the U.S., the BD FACSLyric™ Flow Cytometer is for Research Use Only with BD FACSuite™ Application for up to 12 colors. Not for use in diagnostic or therapeutic procedures.

The BD FACSDuet™ Sample Preparation System is for In Vitro Diagnostic Use. Sample preparation for user-defined protocols and cocktailing functions are for Research Use Only, not for use in diagnostic or therapeutic procedures.

The BD Multitest™ 6-Color TBNK with optional BD Trucount™ Tubes is for In Vitro Diagnostic Use with the BD FACSLyric™ Flow Cytometer, BD FACSCanto™ II Flow Cytometer and BD FACSCanto™ Flow Cytometer and optionally used with BD FACSDuet™ Sample Preparation System.