BD Pharmingen™ Purified Mouse Anti-HPV-16 L1
Clone CAMVIR-1
Preparation And Storage
Recommended Assay Procedures
Immunohistochemistry: For optimal indirect immunohistochemical staining, this antibody should be titrated, such as a 1:10 to 1:50 dilution, and visualized via a three-step staining procedure in combination with polyclonal Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, investigators may be interested in the BD Pharmingen™ Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.
Development References (4)
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Doorbar J, Ely S, Coleman N, Hibma M, Davies DH, Crawford L. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology. 1992; 187(1):353-359. (Biology: Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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McLean CS, Churcher MJ, Meinke J. Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J Clin Pathol. 1990; 43(6):488-492. (Biology: Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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Zhou J, Sun XY, Stenzel DJ, Frazer IH. Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles. Virology. 1991; 185(1):251-257. (Biology: Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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de Villiers EM. Heterogeneity of the human papillomavirus group. J Virol. 1989; 63(11):4898-4903. (Biology: Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required)). View Reference
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