BD Pharmingen™ Purified Rat Anti-Mouse Follicular Dendritic Cell
Clone FDC-M1
Preparation And Storage
Recommended Assay Procedures
Immunohistochemistry: The FDC-M1 clone reactive against mouse follicular dendritic cells is tested for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and small intestine. The antibody stains follicular dendritic cells and their processes. For optimal indirect immunohistochemical staining, the FDC-M1 antibody should be titrated (1-10 to 1-50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and treptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-rat Ig HRP detection kit (Cat. No. 551013) that provides the biotinylated secondary antibody, antibody diluent and the detection system can be used to stain for anti-mouse follicular dendritic cells. Adetailed protocol of the immunohistochemical procedure is enclosed. The clone FDC-M1 is not recommended for formalin-fixed paraffin embedded sections.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Follicular Dendritic Cells (FDC) form a structural network within the lymphoid follicles and are involved in antigen presentation to B cells for generating an immune response and for the maintenance of immunological memory. FDCs are believed to provide signals to B cells to induce their proliferation. In LCMV mediated immunosuppression there is evidence of T-cell dependent destruction of macrophages and follicular dendritic cells. The FDC-M1 antibody recognizes follicular dendritic cells and their dendritic processes.
Development References (3)
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Gray D, Kosco M, Stockinger B. Novel pathways of antigen presentation for the maintenance of memory. Int Immunol. 1991; 3(2):141-148. (Biology: Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen). View Reference
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Kosco, Pflugfelder, and Gray. Follicular dendritic cell-dependent adhesion and proliferation of B cells in vitro. . J Immunol. 1992; 148:2331-2339. (Biology: Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen). View Reference
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Odermatt B, Eppler M, Leist TP, Hengartner H, Zinkernagel RM. Virus-triggered acquired immunodeficiency by cytotoxic T-cell-dependent destruction of antigen-presenting cells and lymph follicle structure. Proc Natl Acad Sci U S A. 1991; 88(18):8252-8256. (Biology: Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen). View Reference
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