BD OptEIA™ Human IL-6 ELISA Kit II

Interleukin-6 (IL-6) is a potent lymphoid cell growth factor which affects B lymphocytes, T lymphocytes, and hybridoma cells. It also affects cytotoxic T cells in combination with other factors such as IL-2 and interferon- γ . Human IL-6 is a 20.5 kD protein containing 184 amino acid residues. The BD OptEIA™ ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance “Recovery” and “Linearity” sections. The BD OptEIA Human IL-6 ELISA Kit II is for the quantitative determination of human IL-6 in serum, plasma, and cell culture supernatant.

Components Quantity  Antibody Coated Wells  2 plates (12 strips x 8 wells per plate)  Detection Antibody  30 mL  Standards:  4 vials, lyophilized  Enzyme Concentrate (250x)  150 µ L  Standard/Sample Diluent  30 mL  ELISA Diluent  12 mL  Wash Concentrate (20x)  100 mL  TMB One-Step Substrate Reagent  30 mL  Stop Solution  13 mL  Plate Sealers  4 each