Panel Design Resources
Multicolor flow cytometry is a powerful technology that enables simultaneous analysis of multiple markers at the single-cell level. With the increase in number of detectable parameters, the design of a multicolor panel can be challenging and requires an understanding of several factors that can influence panel performance:
- Biology—Antigen density and co-expression
- Fluorochrome—Brightness and spillover
- Instrument—Configuration and set-up
The panel design infographic (right) summarizes the flow cytometry panel design journey.
Besides offering innovative instruments, reagents and software, BD Biosciences is committed to providing continuous education on the latest and best panel design approaches to support scientists throughout the full continuum of flow cytometry experiments. Find helpful tools, such as webinars, videos and resources to support multicolor panel design, workflow and experimental analysis. In addition, we also provide several tools, such as Spectrum Viewer, absorption and emission spectra, and buffer compatibility tool.
Multicolor flow cytometry panel design videos
Add A Solid Foundation for Simpler and Smarter Panel Design
A comprehensive overview of panel design fundamentals and different approaches to simplify panel design.
Defining the Objective of Your Experiment
This video is the first in the Scientist to Scientist panel design educational series, designed to provide scientists tips and tricks to overcome common flow cytometry panel design challenges.
Knowing the Biology of Your Experiment
Watch our latest Scientist to Scientist series video to learn some fundamental strategies to improve your flow cytometry panel design.
Understanding Your Flow Cytometry Instrument’s Capabilities for Optimal Panel Design
In this video you will learn how to maximize your flow cytometry instrument’s capabilities for optimized panel design.
Flow Cytometry Instrument Characterization and Set Up for Optimal Panel Design
This video explains how to leverage flow cytometry configurations to help maximize resolution of populations of interest.
Matching Antigens and Fluorochromes for Flow Cytometry Experiments
This video explores practical strategies for matching antigens and fluorochromes for optimal flow cytometry panel design.
Avoiding Resolution Loss When Using Fix and Permeabilization Buffers for Intracellular Staining
In this video you will learn helpful strategies to help prevent loss of resolution due to intracellular fixation and permeabilization buffers.
Understanding and Managing Fluorescence Spillover
In this video you will learn how to manage fluorescence spillover to get the most out of your flow cytometry experiments.
Relationship Between Fluorescence Spillover and Spread
This video will help you understand the relationship between florescence spillover and spread to help you implement the right strategies to resolve your populations of interest.
Minimizing the Impact of Spread in Flow Cytometry Experiments
This video provides strategies to help minimize the impact of spread in flow cytometry experiments to help improve resolution.
Panel Design Workflow for Optimal Fluorochrome-Antigen Matching
This video provides simple strategies for fluorochrome-antigen matching to help prevent the introduction of spread and subsequent resolution loss.
BD Horizon™ Tour Series
This webinar explores concepts, best practices and available tools to help you efficiently navigate multicolor flow cytometry.
Speaker
Andrew D. Bantly
Technical Applications Specialist
Discover a new way of looking at fluorochromes and markers that can maximize the number of parameters achievable on any instrument.
Speaker
Bob Balderas, MBA
VP Biological Sciences & VP Market Development
BD Life Sciences–Biosciences
This webinar offers a comprehensive look at best practices for managing instrument set up to help you maximize your instrument’s parameters while minimizing spillover and resolution loss.
Speaker
Alan Stall
Principle Scientist
BD Life Sciences–Biosciences
This webinar explores the importance of understanding antigen density (the number of cell surface receptors on a cell) in relation to antibody/fluorochrome combinations for optimal panel design.
Speaker
Bob Balderas, MBA
VP Biological Sciences & VP Market Development
BD Life Sciences–Biosciences
This webinar explores the journey to finding what nature is hiding using flow cytometry by understanding the biology of your cells and the relative brightness of fluorochromes.
Speaker
Bob Balderas, MBA
VP Biological Sciences & VP Market Development
BD Life Sciences–Biosciences
BD Harmony Series
This webinar offers viewers practical strategies for designing optimized multicolor flow cytometry panels by addressing frequently asked questions.
Speakers
Bob Balderas, MBA
VP Biological Sciences & VP Market Development
BD Life Sciences–Biosciences
Mirko Corselli, PhD
Global Scientific Content Manager
BD Life Sciences–Biosciences
Rui Gardner, PhD
Head of Flow Cytometry Core Facility
Memorial Sloan Kettering Cancer Center
Yolanda Mahnke, PhD
Founder
FlowKnowHow LLC
This webinar is part of the Harmony Series and offers viewers panel design best practices, with emphasis on spillover and identification of optimal fluorochrome combinations to simplify panel design.
Speaker
Mirko Corselli, PhD
Global Scientific Content Manager
Research Solutions, BD Life Sciences–Biosciences
This webinar explores strategies to help customers create a solid chromatic foundation for multicolor panel design
Speakers
Bob Balderas, MBA
VP Biological Sciences & VP Market Development
BD Life Sciences–Biosciences
Other
This webinar explores strategies for minimizing flow cytometry panel design pain points as well as panel optimization
Speaker
Alan Stall
BD Fellow, Principal Scientist, BD Life Sciences–Biosciences
This webinar offers comprehensive strategies for adding efficiency to the flow cytometry panel design process
Speaker
Anis Larbi, PhD
Head of A*STAR Flow Cytometry Facility, Immuno-monitoring Platform and Principal Investigator, Biology of Aging Program
Singapore Immunology Network (SIgN)
This webinar provides a robust overview on compensation best practices to help achieve your resolution goals.
Speaker
Alan Stall
BD Fellow, Principal Scientist, BD Life Sciences–Biosciences
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Posters
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Tools
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An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
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BD Biosciences Fluorochrome/Laser Reference Chart
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BD Biosciences Fluorochrome Specifications Chart
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BD Biosciences Relative Fluorochrome Brightness Chart
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Human and Mouse CD Marker Handbook
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Mouse Leukocyte Alloantigens Chart
Mahnke YD, Roederer M. Optimizing a multicolor immunophenotyping assay. Clin Lab Med. 2007;27(3): 469–485, v. doi: 10.1016/j.cll.2007.05.002
Mair F, Tyznik A. High-dimensional immunophenotyping with fluorescence-based cytometry: a practical guidebook. Methods Mol Biol. 2019;2032:1-29. doi: 10.1007/978-1-4939-9650-6_1
Nguyen R, Perfetto S, Mahnke YD, Chattopadhyay P, Roederer M. Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A. 2013;83(3):306-315. doi: 10.1002/cyto.a.22251
Roederer M. Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry. 2001;45(3):194-205. doi: 10.1002/1097-0320(20011101)45:3<194::aid-cyto1163>3.0.co;2-c
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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