Introducing BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents
Our next-generation fluorochromes offer:
- Reduced background: significant reduction in monocyte background compared to PE-Cy tandems
- Range of brightness: a range of fluorochromes to support the detection of surface and intracellular markers from a variety of expression levels
- Versatility: compatible with a variety of common fixation and permeabilization systems, viability dyes and anti-coagulants
- Reliable performance: stable reagents that are photostable and offer lot-to-lot consistency
- Panel design flexibility: use RealYellow and RealBlue reagent pairs together on appropriately equipped instruments to build larger panels and for added flexibility when designing panels on both conventional and spectral flow cytometers.
Use the Next-Generation Laser-Specific Flow Cytometry Research Reagents Driven by the New BD Dye Technology Specifically Engineered to Overcome the Challenges of PE Tandems.
Format | Spectral | Conventional | Relative Brightness | Spillover (1-low, 4 – high) | Alternative To |
Blue Laser Line | |||||
RB545 | ✓ |
|
| 1 | Unique position |
RB613 | ✓ | ✓ | 2 | PE-CF594, PE-Dazzle™ 594 or BB630-P2* | |
RB705 | ✓ | ✓ |
| 2 | PerCP-Cy5.5 or BB700* |
RB744 | ✓ | ✓ |
| 1 | BB755-P* |
RB780 | ✓ | ✓ |
| 1 | PE-Cy7 or BB790-P* |
Yellow Laser Line | |||||
RY586 | ✓ | ✓ |
| 1 | PE** |
RY610 | ✓ | ✓ |
| 1 | PE-CF594 or Dazzle™ 594** |
RY703 | ✓ | ✓ |
| 2 | PE-Cy5.5 or StarBright™ Yellow 720** |
RY775 | ✓ | ✓ |
| 2 | PE-Cy7 or StarBright™ Yellow 800** |
*When used with a blue laser on three-laser configuration (B, V, R)
** When used with a yellow-green laser on four or five-laser configuration (B, V, R,YG, or UV, V, B, YG, R)
Compared to PE-Cy tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer reduced background.
BD Horizon™ RB780 Reagents offer reduced background compared to PE-Cy7
RY775 provides improved nonspecific binding.
Compared to PE and PerCP tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer significantly reduced spillover spread.
Compared to PE and PerCP tandems, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer significantly reduced spillover spread.
RB613 shows reduced spillover spread into the corresponding yellow-green detectors.
BD Horizon RealYellow™ 586 Reagents Offer High Resolution with Minimal Cross-Laser Excitation
Unlike select PE tandems such asPE-Cy7, PE-Cy5.5, BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents offer the detection of low-expression surface and intracellular markers.
BD Horizon™ RY703 Reagent resolves low-expression intracellular markers
BD Horizon™ RB780 Reagents support the detection of low-expression intracellular markers.
BD Horizon™ RY775 Reagent resolves intracellular markers
Request a Sample
For more information on all the dyes, go to BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents web page.
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