BD Horizon™ Red 718 Reagents
BD Horizon™ Red 718 (R718) is an innovative small-molecule fluorochrome developed exclusively for BD Biosciences. It provides improved sensitivity and resolution of low-density markers and intracellular targets. R718 is excited by the red laser with an emission maximum of 718 nm and can be detected by the same filter as Alexa Fluor™ 700.
BD Horizon™ Red 718 Reagents are specially designed to serve as alternatives to Alexa Fluor™ 700 or APC-R700.
BD Horizon™ Red 718 Reagents offer:
- Greater brightness and resolving power than Alexa Fluor™ 700 for both surface and intracellular markers
- Reduced background staining compared to Alexa Fluor™ 700 and APC-R700
- Minimal spread into the APC channel
- A broad portfolio of conjugates through BD OptiBuild™ On-Demand Reagents
- Lot-to-lot consistency to reduce risk of data variability
In addition, R718 is compatible with a variety of buffers including BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing Buffers
BD Horizon™ Red 718 Reagents have the potential to enhance your flow cytometry panel's performance.
BD Horizon™ Red 718 Reagents
Two-color comparison staining of IL-17A on stimulated human peripheral blood lymphocytes.
Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate and Ionomycin in the presence of BD GolgiStop™ Protein Transport Inhibitor. The cells were permeabilized with BD Perm/Wash™ Buffer then stained with BD Horizon™ R718 Mouse Anti-Human IL-17A (clone N49-653, A) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human IL-17A (clone N49-653, B) and BD Pharmingen™ PE Mouse Anti-Human CD4 (clone RPA-T4) shown as two-color flow cytometric pseudocolor density plots derived from gated lymphocytes and were analyzed the same day on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software.
Comparison staining of CD4 on human lysed whole blood. A. Lysed whole blood stained with BD Horizon™ R718 Mouse Anti-Human CD4 (clone SK3, red) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human CD4 (clone SK3, grey) shown overlaid as fluorescence histograms derived from a lymphocyte gate. B. SSC vs. fluorescence plots of BD Horizon™ R718 Mouse Anti-Human CD4 (left) or BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human CD4 (right). All data were analyzed on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software.
A. BD Horizon™ R718 FoxP3 is on par with the gold standard BD Pharmingen™ PE FoxP3. Human PBMCs were stained with BD Horizon™ R718 Mouse Anti-Human FoxP3 (clone 259D/C7).
B. BD Phosflow™ Treated Human Control Cells (red) and BD Phosflow™ Untreated Human Control Cells (grey) were stained with BD Phosflow™ R718 Mouse Anti-Stat5 (pY694) (clone 47); treated and untreated control cell overlays shown here are derived from a CD4+ gate.
BD OptiBuild™ On-Demand R718 Reagents Offer Superior Resolution
Comparison of BD Horizon™ R718 Reagents (red), BD OptiBuild™ On-Demand Red 718 Reagents equivalent (blue), vs. BD Pharmingen™ Alexa Fluor™ 700 (orange) using surface markers mouse anti-human CD3 (left, clone UCHT1) or rat anti-mouse CD8a (right, clone 53-6.7)
Featured Reagents
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| Reagent Specificity | Species | Clone | Isotype | Size | Catalog Number |
| BCL-6 | Human | K112-91 | Mouse IgG1, κ | 100 Tests | 566979 |
| CD3 | Human | UCHT1 | Mouse IgG1, κ | 100 Tests | 566953 |
| 25 Tests | 566954 | ||||
| CD4 | Human | SK3 | Mouse IgG1, κ | 100 Tests | 566930 |
| 25 Tests | 566931 | ||||
| CD11c | Human | B-ly6 | Mouse IgG1, κ | 100 Tests | 566932 |
| 25 Tests | 566933 | ||||
| CD19 | Human | SJ25C1 | Mouse IgG1, κ | 100 Tests | 566946 |
| 25 Tests | 566947 | ||||
| CD279 (PD-1) | Human | EH12.1 | Mouse IgG1, κ | 100 Tests | 566974 |
| 25 Tests | 566975 | ||||
| FoxP3 | Human | 259D/C7 | Ms IgG1 | 100 Tests | 566935 |
| 25 Tests | 566936 | ||||
| IL-17A | Human | N49-653 | Mouse IgG1, κ | 100 Tests | 566938 |
| Stat5 (pY694) | Human | 47/Stat5 (pY694) | Mouse IgG1, κ | 100 Tests | 566977 |
| 25 Tests | 566978 | ||||
| CD107a | Mouse | 1D4B | Rat IgG2a, κ | 50 ug | 566986 |
| CD4 | Mouse | RM4-5 | Rat IgG2a, k | 50 ug | 566939 |
| CD8a | Mouse | 53-6.7 | Rat IgG2a, κ | 50 ug | 566985 |
| CD13 | Mouse | R3-242 | Rat IgG1 | 50 ug | 566982 |
| Ly-6C | Mouse | AL-21 | Rat IgG2a, κ | 50 ug | 566987 |
Switch to BD Horizon™ Red 718 Reagents to achieve your resolution goals.
Available ReagentsAlexa Fluor® 700 is a trademark of Thermo Fisher Scientific.
Class 1 Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD, the BD Logo, Cytofix/Cytoperm, FlowJo, GolgiStop, Horizon, LSRFortessa, OptiBuid, Pharmingen and Phosflow are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2020 BD. All rights reserved.
